October 13th, 2008
•The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.
Related Videos
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
Blood Collection for Biochemical Analysis in Adult Zebrafish
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Sampling Blood from the Lateral Tail Vein of the Rat
Repeated Blood Collection for Blood Tests in Adult Zebrafish
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved