Name: assessing burrowing, nest construction, and hoarding in mice
Uploaded: 2012-01-05T12:00:00.000+00:00
Duration: 8 min 23 s
Description: Watch this Scientific Journal Video about Assessing Burrowing, Nest Construction, and Hoarding in Mice at JoVE.com

ويلكوم ترست لتوفير التمويل فتح الوصول إلى جامعة أكسفورد. روبرت ديكون هو عضو في مجموعة أكسفورد OXION ، بتمويل من منحة ويلكوم ترست WT084655MA.

<ol>
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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Burrowing:+A+sensitive+behavioural+assay,+tested+in+five+species+of+laboratory+rodents.">Burrowing: A sensitive behavioural assay, tested in five species of laboratory rodents.</a> Behav. Brain. Res. 200, 128-133 (2009).</li><li>Deacon, R. M. J., Bannerman, D. M., Kirby, B. P., Croucher, A., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+cytotoxic+hippocampal+lesions+in+mice+on+a+cognitive+test+battery.">Effects of cytotoxic hippocampal lesions in mice on a cognitive test battery.</a> Behav. Brain. Res. 133, 57-68 (2002).</li><li>Deacon, R. M. J., Brook, R. C., Meyer, D., Haeckel, O., Ashcroft, F. M., Miki, T., Seino, S., Liss, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+phenotyping+of+mice+lacking+the+KATP+channel+subunit+Kir6.2.">Behavioral phenotyping of mice lacking the KATP channel subunit Kir6.2.</a> Physiol. Behav. 87, 723-733 (2006).</li><li>Deacon, R. M. J., Croucher, A., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hippocampal+cytotoxic+lesion+effects+on+species-typical+behaviors+in+mice.">Hippocampal cytotoxic lesion effects on species-typical behaviors in mice.</a> Behav. Brain. Res. 132, 203-213 (2002).</li><li>Deacon, R. M. J., Penny, C., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+medial+prefrontal+cortex+cytotoxic+lesions+in+mice.">Effects of medial prefrontal cortex cytotoxic lesions in mice.</a> Behav. Brain. Res. 139, 139-155 (2003).</li><li>Deacon, R. M. J., Raley, J. M., Perry, V. H., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Burrowing+into+prion+disease.">Burrowing into prion disease.</a> Neuroreport. 12, 2053-2057 (2001).</li><li>Deacon, R. M. J., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hippocampal+lesions,+species-typical+behaviours+and+anxiety+in+mice.">Hippocampal lesions, species-typical behaviours and anxiety in mice.</a> Behav. Brain. Res. 156, 241-249 (2005).</li><li>Deacon, R. M. J., Thomas, C. L., Rawlins, J. N. P., Morley, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparison+of+the+behavior+of+C57BL/6+and+C57BL/10.">A comparison of the behavior of C57BL/6 and C57BL/10.</a> Behav. Brain. Res. 179, 239-247 (2007).</li><li>Guenther, K., Deacon, R. M. J., Perry, V. H., Rawlins, J. N. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+behavioural+changes+in+scrapie-affected+mice+and+the+influence+of+dapsone.">Early behavioural changes in scrapie-affected mice and the influence of dapsone.</a> Eur. J. Neurosci. 14, 401-409 (2001).</li><li>Mallucci, G. R., White, F. a. r. m. e. r., Dickinson, M., Khatun, A., Powell, H., Brandner, A. D., Jefferys, S., R, J. G., Collinge, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeting+Cellular+Prion+Protein+Reverses+Early+Cognitive+Deficits+and+Neurophysiological+Dysfunction+in+Prion-Infected+Mice.">Targeting Cellular Prion Protein Reverses Early Cognitive Deficits and Neurophysiological Dysfunction in Prion-Infected Mice.</a> Neuron. 53, 325-335 (2007).</li><li>Russell, W. M. S., Burch, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The principles of humane experimental technique. , (1959).</li><li>Schmid-Holmes, S., Drickamer, L. C., Robinson, A. S., Gillie, L. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Burrows+and+burrow+cleaning+behaviour+of+house+mice.">Burrows and burrow cleaning behaviour of house mice.</a> Am. Mid. Nat. 146, 53-62 (2001).</li><li>Teeling, J. L., Felton, L. M., Deacon, R. M. J., Cunningham, C., Rawlins, J. N. P., Perry, V. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sub-pyrogenic+systemic+inflammation+impacts+on+brain+and+behavior,+independent+of+cytokines.">Sub-pyrogenic systemic inflammation impacts on brain and behavior, independent of cytokines.</a> Brain Behav. Immun. 21, 836-850 (2007).</li></ol>

الإعلان عن أي تضارب في المصالح.

<html> هذه التجارب هي بسيطة للغاية لتشغيل ولكن تأثير أحجام كبيرة جدا ، مما يجعلها حساسة للغاية للعلاجات ، والاختلافات السلالة ، الخ وهي تتفق مع الجانب &quot;تنقيح&quot; من راسيل وبورتش في 3 روبية &quot;18. في الواقع ، والأداء الجيد في اختبارات مثل تختبئ ويبدو أن يكون مؤشرا جيدا للصحة العامة في الحيوانات. وبالتالي يمكن أن يكون هذا اختبارا جيدا لتقييم الرفق بالحيوان. ومن المعروف جيدا أهمية تخصيب البيئية للقوارض المختبرات في هذه الأيام. توفير مواد التعشيش الآن ممارسة معتادة في منازل الكثير من الحيوانات ، وهذا أمر من السهل القيام به. الحماس الذي يجعل تختبئ الفئران تحفر لتخصيب ممتازة يحتمل ، ولكن للأسف الذاتي &quot;إعادة&quot; الجحر الذي سيكون وسيلة عملية لتوفير هذا لم يتم حتى الآن نموا. إذا كان العثور على الفئران اكتناز إثراء لم يتحدد بعد. _content &quot;&gt; عيب واحد هو أن البيانات التي تولدها هذه الاختبارات بصفة عامة غير المعلمية (وليس التمويه في التوزيع) ، وهناك حاجة لذلك غير مناسب بارامترية إحصاءات لتحليلها ، على سبيل المثال اختبار مان ويتني U للمقارنات البشرى ، كروسكال - اليس ANOVA لمجموعة البيانات المتعددة. إذا تم استخدام تكرار التصميمات وتدابير ، لأنه ليس هناك أي اختبار ANOVA مناسبة لغير بارامترية البيانات ، يمكن أن تتحول هذه النتائج ، مثلا عن طريق الجذر التربيعي أو إجراءات تسجيل لجعلها مطابقة لتوزيع أكثر طبيعية . يمكن للقياسات الجهاز يكون مختلفا قليلا الى تلك المحددة في النص (مفيدة للالمجربون الولايات المتحدة) ولكن ينبغي تجنب خلافات واسعة. ومع ذلك ، في بعض الحالات ، قد يجد المجربون أخرى الانشاءات المختلفة تعمل بشكل أفضل من تلك التي وصفها هنا. كما لجميع القياسات السلوكية ، ينبغي اختباره على الفئران لمعرفة ما اذا مستويات نشاطهم طبيعية ، وسوف يكون الخمول الواضح detrimental تأثير على الأنواع التقليدية السلوكيات. وكانت مستويات نشاطهم عندما سكرابي الفئران المصابة كانت تختبئ تظهر ضعف وتداخل ، العادي (اختبار في جهاز حقل مفتوح). في الواقع ، وهي فترة من النشاط الزائد يحدث حوالي 2-4 أسابيع بعد ضعاف أولا هذه السلوكيات الأنواع النموذجية 2. وضعف قوية تنتجها الآفات الحصينية على تختبئ ، تداخل واكتناز ، والتي هي من الأنواع التقليدية ، السلوكيات ، ويكمل العاهات في التعلم المكاني والتي هي من هذا القبيل memory9 راسخة آثار هذه الآفات. على ما يبدو أن يكون معادلا لعاهات في أنشطة الحياة اليومية من سمات ميلادي 14. قد تكون هذه الاختبارات الأنواع النموذجية تثبت جيدا لتكون ذات فائدة كبيرة في الفحص قبل السريرية لعلاج لمرض الزهايمر والأمراض العصبية الأخرى 14. في كثير من اضطرابات الدماغ البشري قدرة خسارة أو تخفيض لأداء عشية العاديryday مهام لا تقل أهمية عن غيرها من الميزات لهذا المرض مثل ضعف الادراك. خاصة ، وهذا قد يتطلب إشراك فقدان مقدمي الرعاية والاجتماعية والاقتصادية هذا عبء كبير في كثير من الأحيان تفرض.

assessing burrowing, nest construction, and hoarding in mice

تدهور في القدرة على تنفيذ &quot;نشاطات الحياة اليومية&quot; (ADL) هو علامة مبكرة لمرض الزهايمر (ميلادي). الفحص قبل السريرية للعلاجات السلوكية الممكنة لميلادي الى حد كبير في الوقت الراهن تركز على التجارب المعرفية ، مما يتطلب في كثير من الأحيان معدات باهظة الثمن والكثير من الوقت مجرب. ومع ذلك ، والذاكرة العرضية الإنسان (الجانب الأكثر تضررا من الذاكرة في ميلادي) مختلفة إلى الذاكرة القوارض ، والذي يبدو إلى حد كبير غير العرضية. ولذلك طرق الفحص الحالية للعلاجات جديدة لميلادي ميلادي في القوارض من المستبعد أن ينجح في جوهرها. ومن شأن اتباع نهج جديد لفحص ما قبل السريرية تكون لتوصيف ADL من الفئران. مصادفة ، فقد تم مؤخرا العديد من هذه المقايسات المتقدمة في جامعة أكسفورد ، وهنا يتم تقديم الثلاثة الأكثر حساسية وتتميز كذلك. وكان أول من طور تختبئ في أكسفورد 13. تطورت فإنه من الحاجة إلى تطوير نموذج اكتناز الماوس. معظم نشرمطلوب نماذج الطبعة اكتناز القوارض تكون مرتبطة مصدر الغذاء بعيدة إلى قفص المنزل بواسطة ممر يربط. وهذا يتضمن تعديل قفص المنزل فضلا عن جعل ماوس إثبات مرور وربط مصدر الغذاء. حتى ما إذا كان يعتبر انه سيكون من الممكن وضع مصدر الغذاء داخل القفص. وكانت كريات الغذاء وجد أنه إذا وضعت وعاء على الأرض وإفراغها من قبل صباح اليوم التالي.. ، ، ومع ذلك ، أودعت ببساطة في كومة عند مدخل حاوية ، بدلا من وضعها في مكان منفصل بعيدا عن الحاوية ، كما يمكن أن يتوقع إذا كانت الفئران اكتناز حقا لهم. وأظهرت وثيقة التفتيش أن الفئران كانوا يقومون بحفر (&quot;تختبئ&quot;) الحركات ، التي لا تحمل الكريات في أفواههم إلى مكان المحددة كما لو اكتناز حقا لهم. 6 الكريات الغذائية لا تشكل الركيزة الأساسية للنقب ؛ الفئران أنابيب فارغة ومليئة بالحصى والرمل ، والفراش حتى المتسخة من القفص الخاصة بهم. أكثرأكثر ، فإنها فارغة أنبوب الكامل حتى لو وضعت فارغ بجانبه 8. تداخل العديد من البروتوكولات موجودة في الأدب. في أكسفورد تمثل أحد يبسط الإجراءات وسجل لديها نظام واضح المعالم لجودة عش 5. وقد وضعت في وقت لاحق واكتناز في النموذج الذي تم تكييفه للفئران ، بدلا من العودة الى اكتناز القفص الوطن الحقيقية ، للعيش في &quot;قاعدة الداخل&quot; من جهاز الاحتكار. كان على علاقة هذه القاعدة مقرا لأنبوب مصنوع من اسلاك ، ونهاية البعيدة التي تحتوي على مصدر الغذاء. أثبت هذا الترتيب لانتاج اكتناز السلوك الجيد ، طالما تم تكييف الفئران على العيش في &quot;قاعدة الوطن&quot; خلال النهار ، ويسمح فقط لإدخال أنبوب اكتناز ليلا.

Watch this Scientific Journal Video about Assessing Burrowing, Nest Construction, and Hoarding in Mice at JoVE.com

Assessing Burrowing, Nest Construction, and Hoarding in Mice

نقب ، واكتناز وتداخل الأنواع النموذجية الأنشطة التي تؤدي بسهولة الفئران في المختبر. توضح هذه المقالة كيف يمكن أن بسهولة وبثمن بخس المقررة. هذه البروتوكولات هي حساسة للغاية لسلالة الفأر ، والآفات والأمراض في الدماغ. وعلاوة على ذلك فهي تشكل &quot;تخصيب البيئية&quot; للفئران ، وتجسد &quot;تنقيح&quot; جانب من جوانب &quot;3 روبية&quot; 18.

تقييم الاختراق ، عش البناء ، واكتناز في الفئران

Deterioration in the ability to perform "Activities of daily living" (ADL) is an early sign of Alzheimer's disease (AD). Preclinical behavioural screening ...

assessing-burrowing-nest-construction-and-hoarding-in-mice

Research

JoVE Journal

Neuroscience

32.0K Views.  University of Oxford. نقب ، واكتناز وتداخل الأنواع النموذجية الأنشطة التي تؤدي بسهولة الفئران في المختبر. توضح هذه المقالة كيف يمكن أن بسهولة وبثمن بخس المقررة. هذه البروتوكولات هي حساسة للغاية لسلالة الفأر ، والآفات والأمراض في الدماغ. وعلاوة على ذلك فهي تشكل "تخصيب البيئية" ل?...

Video: Assessing Burrowing, Nest Construction, and Hoarding in Mice

Marble Burying and Nestlet Shredding as Tests of Repetitive, Compulsive-like Behaviors in Mice

Obsessive-compulsive disorder (OCD) and autism spectrum disorders (ASD) are serious and debilitating psychiatric conditions and each constitutes a significant public health concern, particularly in children. Both of these conditions are highlighted by the repeated expression of meaningless behaviors. Individuals with OCD often show checking, frequent hand washing, and counting. Children with ASDs also engage in repetitive tapping, arm or hand flapping, and rocking. These behaviors can vary widely in intensity and frequency of expression. More intense forms of repetitive behaviors can even result in injury (e.g.&#160;excessive grooming, hand washing, and self-stimulation). These behaviors are therefore very disruptive and make normal social discourse difficult. Treatment options for repetitive behaviors in OCD and ASDs are somewhat limited and there is great interest in developing more effective therapies for each condition. Numerous animal models for evaluating compulsive-like behaviors have been developed over the past three decades. Perhaps the animal models with the greatest validity and ease of use are the marble burying test and the nestlet shredding test. Both tests take advantage of the fact that the target behaviors occur spontaneously in mice. In the marble burying test, 20 marbles are arrayed on the surface of clean bedding. The number of marbles buried in a 30 min session is scored by investigators blind to the treatment or status of the subjects. In the nestlet shredding test, a nestlet comprised of pulped cotton fiber is preweighed and placed on top of cage bedding and the amount of the nestlet remaining intact after a 30 min test session is determined. Presently, we describe protocols for and show movie documentation of marble burying and nestlet shredding. Both tests are easily and accurately scored and each is sensitive to small changes in the expression of compulsive-like behaviors that result from genetic manipulations, disease, or head injury.

Disease, head injury, genetic modifications, and treatment of mice with drugs can have profound effects on behavior. Utilizing well-characterized and validated approaches such as marble burying and nestlet shredding, compulsive-like behaviors can be documented accurately in mice as models of human obsessive-compulsive disorder and autism spectrum disorder.

Obsessive-compulsive disorder (OCD) and autism spectrum disorders (ASD) are serious and debilitating psychiatric conditions and each constitutes a ...

Behavior

Nest Building as an Indicator of Health and Welfare in Laboratory Mice

The minimization and alleviation of suffering has moral and scientific implications. In order to mitigate this negative experience one must be able to identify when an animal is actually in distress. Pain, illness, or distress cannot be managed if unrecognized. Evaluation of pain or illness typically involves the measurement of physiologic and behavioral indicators which are either invasive or not suitable for large scale assessment. The observation of nesting behavior shows promise as the basis of a species appropriate cage-side assessment tool for recognizing distress in mice. Here we demonstrate the utility of nest building behavior in laboratory mice as an ethologically relevant indicator of welfare. The methods presented can be successfully used to identify thermal stressors, aggressive cages, sickness, and pain. Observation of nest building behavior in mouse colonies provides a refinement to health and well-being assessment on a day to day basis.

We demonstrate the utility of nest building behavior in laboratory mice as an indicator of welfare. Nest scoring is a sensitive technique that is altered by temperature, illness, and aggression. The time to integrate into nest test (TINT) is a simple cage-side assessment that can detect postoperative pain.

The minimization and alleviation of  suffering has moral and scientific implications. In order to mitigate this  negative experience one must be able to ...

Assessment of Social Interaction Behaviors

Social interactions are a fundamental and adaptive component of the biology of numerous species. Social recognition is critical for the structure and stability of the networks and relationships that define societies. For animals, such as mice, recognition of conspecifics may be important for maintaining social hierarchy and for mate choice 1.
A variety of neuropsychiatric disorders are characterized by disruptions in social behavior and social recognition, including depression, autism spectrum disorders, bipolar disorders, obsessive-compulsive disorders, and schizophrenia. Studies of humans as well as animal models (e.g., Drosophila melanogaster, Caenorhabditis elegans, Mus musculus, Rattus norvegicus) have identified genes involved in the regulation of social behavior 2. To assess sociability in animal models, several behavioral tests have been developed (reviewed in 3). Integrative research using animal models and appropriate tests for social behavior may lead to the development of improved treatments for social psychopathologies.
The three-chamber paradigm test known as Crawley's sociability and preference for social novelty protocol has been successfully employed to study social affiliation and social memory in several inbred and mutant mouse lines (e.g. 4-7). The main principle of this test is based on the free choice by a subject mouse to spend time in any of three box's compartments during two experimental sessions, including indirect contact with one or two mice with which it is unfamiliar. To quantitate social tendencies of the experimental mouse, the main tasks are to measure a) the time spent with a novel conspecific and b) preference for a novel vs. a familiar conspecific. Thus, the experimental design of this test allows evaluation of two critical but distinguishable aspects of social behavior, such as social affiliation/motivation, as well as social memory and novelty. "Sociability" in this case is defined as propensity to spend time with another mouse, as compared to time spent alone in an identical but empty chamber 7. "Preference for social novelty" is defined as propensity to spend time with a previously unencountered mouse rather than with a familiar mouse 7. This test provides robust results, which then must be carefully analyzed, interpreted and supported/confirmed by alternative sociability tests. In addition to specific applications, Crawley's sociability test can be included as an important component of general behavioral screen of mutant mice.

Here we describe a detailed protocol for examination of sociability in mice by using Crawley's sociability and preference for social novelty test. We describe the advantages and possible applications for this procedure, including critical details important for correct interpretation of the results.

تقييم سلوك التفاعل الاجتماعي

Social interactions are a fundamental and adaptive component of the biology of numerous species. Social recognition is critical for the structure and ...

A Behavioral Assay for Investigating the Role of Spatial Memory During Instinctive Defense in Mice

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often stereotyped actions elicited in response to innately aversive sensory stimuli, but their success requires enough flexibility for adapting to different spatial environments, which can change rapidly. Here, we describe a behavioral assay to evaluate the influence of learned spatial knowledge on defensive behaviors in mice. We have adapted the widely used Barnes maze spatial memory assay to investigate how mice navigate to a shelter during escape responses to innately aversive sensory stimuli in a novel environment, and how they adapt to acute changes in the environment. This new assay is an ethological paradigm that does not require training and exploits the natural exploration patterns and navigation strategies in mice. We propose that the set of protocols described here are a powerful means of studying goal-directed behaviors and stimulus-triggered navigation, which should be of interest to both the fields of instinctive behaviors and spatial memory.

This protocol describes a behavioral assay, based on the Barnes maze test, to study how instinctive defensive actions are modified by the knowledge of spatial environment.

تحليل السلوكي للتحقيق في دور الذاكرة المكانية خلال الدفاع الغريزي في الفئران

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often ...

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

In keeping with the 3R Principle (Replacement, Reduction, Refinement) developed by Russel and Burch, scientific research should use alternatives to animal experimentation whenever possible. When there is no alternative to animal experimentation, the total number of laboratory animals used should be the minimum needed to obtain valuable data. Moreover, appropriate refinement measures should be applied to minimize pain, suffering, and distress accompanying the experimental procedure. The categories used to classify the degree of pain, suffering, and distress are non-recovery, mild, moderate, or severe (EU Directive 2010/63). To determine which categories apply in individual cases, it is crucial to use scientifically sound tools.
The well-being-assessment protocol presented here is designed for procedures during which general anesthesia is used. The protocol focuses on home cage activity, the Mouse Grimace Scale, and luxury behaviors such as burrowing and nest building behavior as indicators of well-being. It also uses the free exploratory paradigm for trait anxiety-related behavior. Fecal corticosterone metabolites as indicators of acute stress are measured over the 24-h post-anesthetic period.
The protocol provides scientifically solid information on the well-being of mice following general anesthesia. Due to its simplicity, the protocol can easily be adapted and integrated in a planned study. Although it does not provide a scale to classify distress in categories according to the EU Directive 2010/63, it can help researchers estimate the degree of severity of a procedure using scientifically sound data. It provides a way to improve the assessment of well-being in a scientific, animal-centered manner.

We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.

تقييم منهجي لرفاهية في الفئران للإجراءات باستخدام التخدير العام

In keeping with the 3R Principle (Replacement, Reduction, Refinement) developed by Russel and Burch, scientific research should use alternatives to animal ...

Nest Building Behavior as an Early Indicator of Behavioral Deficits in Mice

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents synthetic and/or natural materials (such as twine, tissue, cotton, paper, and hay) as a gauge of their overall well-being and as an ancillary assessment to predict the possible decline in cognition. Typically, changes in nesting behaviors, such as failure to create a nest, indicate a change in health or welfare. In addition, nesting behavior is sensitive to many environmental and physiological challenges, as well as many genetic mutations underlying pathological disease states. The following protocol describes a nesting behavior paradigm that explores the usage of four types of nesting material. In addition, the protocol utilizes intraclass correlations to demonstrate that inter-rater reliability is higher when nests are constructed out of shredded paper compared to other common nesting materials such as cotton squares, paper twists, and soft cob bedding. The chosen methodology and statistical considerations (i.e., intraclass correlation) for this assay may be of interest for those conducting experiments assessing the quality of living of mice.&#160;

Here, we present a protocol to quantify nest building behavior in mice, which is known to be impaired in several neurological disorders and diseases. This protocol examines the utility of four materials and offers the opportunity to quantify the rater agreement in scoring, improving the validity and reliability of the assay.

سلوك بناء العش كمؤشر مبكر للعجز السلوكي في الفئران

Nest building is an innate behavior in male and female rodents, even when raised in laboratory settings. As such, many researchers provide rodents ...

Assessment of Mouse Judgment Bias through an Olfactory Digging Task

Judgment biases (JB) are differences in the way that individuals in positive and negative affective/emotional states interpret ambiguous information. This phenomenon has long been observed in humans, with individuals in positive states responding to ambiguity 'optimistically' and those in negative states instead showing 'pessimism'. Researchers aiming to assess animal affect have taken advantage of these differential responses, developing tasks to assess judgment bias as an indicator of affective state. These tasks are becoming increasingly popular across diverse species and fields of research. However, for laboratory mice, the most widely used vertebrates in research and a species heavily relied upon to model affective disorders, only one JB task has been successfully validated as sensitive to changes in affective state. Here, we provide a detailed description of this novel murine JB task, and evidence of its sensitivity to mouse affect. Though refinements are still necessary, assessment of mouse JB opens the door for answering both practical questions regarding mouse welfare, and fundamental questions about the impact of affective state in translational research.

This article provides a detailed description of a novel mouse judgment bias protocol. Evidence of this olfactory digging task's sensitivity to affective state is also demonstrated and its utility across diverse research fields is discussed.

تقييم تحيز حكم الفأر من خلال مهمة الحفر الشمي

Judgment biases (JB) are differences in the way that individuals in positive and negative affective/emotional states interpret ambiguous information. This ...

<meta charset="utf8"></head><body>المقايسات السلوكية لاضطراب طيف التوحد في نماذج الفئران

Strategies for Assessing Autistic-Like Behaviors in Mice

Autism spectrum disorder (ASD) is a neurobiologically complex condition with a heterogeneous genetic etiology. Clinically, ASD is diagnosed by social communication impairments and restrictive or repetitive behaviors, such as hand flapping or lining up objects. These behavioral patterns can be reliably observed in mouse models with ASD-linked genetic mutations, making them highly useful tools for studying the underlying cellular and molecular mechanisms in ASD. Understanding how genetic changes affect the neurobiology and behaviors observed in ASD will facilitate the development of novel targeted therapeutic compounds to ameliorate core behavioral impairments. Our lab has employed several protocols encompassing well-described training and testing procedures that reflect a wide range of behavioral deficits related to ASD. Here, we detail two assays to study the core features of ASD in mouse models: self-grooming (a measure of repetitive behavior) and the three-chamber social interaction test (a measure of social interaction approach and preference for social novelty).

<meta charset="utf8"></head><body>استراتيجيات تقييم السلوكيات الشبيهة بالتوحد في الفئران

Rodent models are valuable tools for studying core behaviors related to autism spectrum disorder (ASD). In this article, we expound on two behavioral tests for modeling the core features of ASD in mice: self-grooming, which assesses repetitive behavior, and the three-chamber social interaction test, which documents social impairments.

استراتيجيات تقييم السلوكيات الشبيهة بالتوحد في الفئران

Autism spectrum disorder (ASD) is a neurobiologically complex condition with a heterogeneous genetic etiology. Clinically, ASD is diagnosed by social ...

تقييم الاختراق ، عش البناء ، واكتناز في الفئران

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Marble Burying and Nestlet Shredding as Tests of Repetitive, Compulsive-like Behaviors in Mice

Nest Building as an Indicator of Health and Welfare in Laboratory Mice

تقييم سلوك التفاعل الاجتماعي

تحليل السلوكي للتحقيق في دور الذاكرة المكانية خلال الدفاع الغريزي في الفئران

تقييم منهجي لرفاهية في الفئران للإجراءات باستخدام التخدير العام

سلوك بناء العش كمؤشر مبكر للعجز السلوكي في الفئران

تقييم تحيز حكم الفأر من خلال مهمة الحفر الشمي

استراتيجيات تقييم السلوكيات الشبيهة بالتوحد في الفئران

استراتيجيات تقييم السلوكيات الشبيهة بالتوحد في الفئران

استراتيجيات تقييم السلوكيات الشبيهة بالتوحد في الفئران