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فحوصات البيوكيميائية لتحليل أنشطة ATP التي تعتمد على لونين إعادة عرض الانزيمات

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

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Chapters in this video

0:05

Title

1:15

ATP-dependent Nucleosome Remodeling Assays

2:55

ATP-dependent Nucleosome Sliding Assays

5:11

DNA- and Nucleosome-dependent ATPase Assays

7:15

Defining Biochemical Activities of the INO80 Complex and INO80 Subcomplexes

9:17

Conclusion

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