Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

11.0K Views

14:46 min

May 28th, 2015

DOI :

10.3791/52539-v

May 28th, 2015


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HiPSC

Chapters in this video

0:05

Title

1:54

Design of Targeting Vectors

2:41

Design and Vector Construction of sgRNAs for CRISPR/Cas9 System

5:20

Design and Construction of Cas9n Double Nickase sgRNA

8:06

Evaluation of sgRNAs by T7 Endonuclease1

11:03

In Silico Prediction of Potential Off Target Sites

12:32

Results: The T7E1 Assay is Used to Evaluate sgRNAs Prior to Transfecting hiPSCs

13:56

Conclusion

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