An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

9.9K Views

•

07:43 min

•

August 23rd, 2016

DOI :

10.3791/54018-v

August 23rd, 2016

•

Martin H. M. Sailer¹, Durga Sarvepalli², Catherine Brégère³, Urs Fisch³, Marin Guentchev⁴, Michael Weller⁶, Raphael Guzman³, Bernhard Bettler¹, Arkasubhra Ghosh*², Gregor Hutter*⁵

¹Dept. of Biomedicine, Pharmacenter, University of Basel, ²Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, ³Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, ⁴Department of Neurosurgery, Klinikum Idar-Oberstein, ⁵Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, ⁶Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich

Summary

Explore More Videos

Keywords Neural Stem Cell

Epithelial To Mesenchymal Transition

Chapters in this video

0:05

Title

1:11

Cortical Subventricular Zone (SVZ) Dissection and Preparation

4:28

Explant Culture Preparation

5:43

Results: Representative Quantitative Migration Assessment

6:28

Conclusion

Related Videos

06:09

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

14.0K Views

08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

10.2K Views

09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

8.1K Views

12:21

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

10.5K Views

12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

10.2K Views

06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

9.2K Views

11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

8.7K Views

09:29

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

10.7K Views

12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

7.1K Views

06:57

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

8.9K Views