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Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

36.2K Views

12:40 min

June 6th, 2016

DOI :

10.3791/54204-v

June 6th, 2016


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Mesenchymal Stem Cells

Chapters in this video

0:00

Title

1:00

Methodologies

2:20

Introduction

3:12

Tissues to be Harvested

3:53

Preparation of the Placenta and the Placental Anatomy

4:40

Dissection of the Decidua (D) Tissue

5:40

Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue

6:36

Mincing and Enzymatic Digestion of the Placental Tissue

7:25

Collection of Mononuclear Cells and Plating into Flasks

9:33

Post Isolation - Cell Growth and Expansion

9:57

Representative Results: Culture MSC and Assay Cells for MSC Characteristics

12:07

Conclusion

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