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Growing Mycobacterial Biofilm as a Model to Study Antimicrobial Resistance

445 Views

•

04:26 min

•

July 12th, 2024

DOI :

10.3791/66607-v

July 12th, 2024

•

Kaushik Poddar¹, Amitesh Anand¹

¹Department of Biological Sciences, Tata Institute of Fundamental Research

Transcript

Our research focuses on bacterial metabolism, especially energy metabolism, which is crucial for growth and survival. Despite extensive biochemical and structural data, systems level understanding and adaptive mechanisms remain unclear. We aim to deepen this knowledge and to address antimicrobial resistance.

Our protocol enhances the physiological relevance of lab experiments by using biofilms, which mimics the natural bacterial growth better than the planktonic methods. Adapting microbacterial biofilms will offer a great system to understand the gene's lifestyle, and it'll also offer a screening system for antimicrobials. We are not the first ones to produce mycobacterial biofilms.

There are several groups using this as a model. Our motivation behind producing this protocol was to simplify the procedures as much as possible so many labs can adapt this system as a model.

Summary

This protocol describes a robust method for developing pellicle biofilm. The method is scalable to different culture volumes, allowing easy adoption for various experimental objectives. The method's design enables qualitative or quantitative assessment of the biofilm-forming potential of several mycobacterial species.

Explore More Videos

Mycobacterial Biofilm

Antimicrobial Resistance

Bacterial Metabolism

Energy Metabolism

Biofilm Formation

Microbial Communities

Planktonic Methods

Cultivation Techniques

Mycobacterium Smegmatis

Biofilm Dynamics

Transcriptional Profiles

Experimental Methods

Screening System

Chapters in this video

0:00

Introduction

1:06

Culture of Mycobacterium smegmatis for Biofilm Production

2:24

Setting Up and Estimating the Pellicle Biofilm Development in Mycobacterium smegmatis Cultures

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