Preparing the Eggs for Incubation and Opening the Eggshell on Day 3 of Chick Embryo Development (ED)
2:38
Grafting Frozen-Thawed Human Ovarian Tissue to the CAM and Harvesting the Grafts
3:33
Results: Development of a CAM Xenografting Model for Human Ovarian Tissue
4:37
Conclusion
Transcript
The use of CAM to observe early changes after ovarian tissue transplantation has a significant advantage over in vitro models, since it allows the investigation of mechanism affecting the early vascularization and follicle loss process. This techn
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Here, we describe a protocol for developing a chick chorioallantoic membrane (CAM) xenografting model for human ovarian tissue and demonstrate the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period.