Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
2.7K Views
•
06:36 min
•
September 8th, 2021
DOI :
September 8th, 2021
•Josselin Rigaill*1,2, Estelle Audoux*1, Killian Rodriguez1, Aurélien Peyron1, Philippe Berthelot1,3, Jérôme Josse4,5, Frédéric Laurent4,5, Robin Caire1, Paul O. Verhoeven1,2
1CIRI, Centre International de Recherche en Infectiologie, GIMAP team, University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, 2Department of Infectious Agents and Hygiene, University Hospital of St-Etienne, St-Etienne, France, 3Department of Infectious Diseases, University Hospital of St-Etienne, St-Etienne, France, 4CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team, University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, 5Department of Bacteriology, Institute for Infectious Agents, Hospices Civils de Lyon, Lyon, France
Chapters in this video
0:05
Introduction
0:41
Determination of Cell Density and Viability
1:33
Preparation of the Bacterial Suspension
2:32
Cell Inoculation
3:03
Quantification of Intracellular Bacteria with Improved Enzyme Protection Assay (iEPA)
4:44
Results: Intracellular Staphylococcus aureus Load in Epithelial Cells
5:54
Conclusion
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