Norwegian University of Science and Technology

5 ARTICLES PUBLISHED IN JoVE

Environment

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge

Simon Felz¹, Salah Al-Zuhairy¹, Olav Andreas Aarstad², Mark C.M. van Loosdrecht¹, Yue Mei Lin¹

¹Department of Biotechnology, Delft University of Technology, ²Department of Biotechnology, Norwegian Biopolymer Laboratory (NOBIPOL), Norwegian University of Science and Technology

The protocol provides a methodology to solubilize aerobic granular sludge in order to extract alginate-like extracellular polymers (ALE).

Neuroscience

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Giulia Quattrocolo¹, Maria Isaac², Yajun Zhang², Timothy J. Petros²

¹Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, ²Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health

Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups.

Neuroscience

Geir Ogrim^1,2,3, Juri D. Kropotov^4,5

¹Neuropsychiatric Team, Åsebråten Outpatient Clinic, Østfold Hospital Trust, ²Institute of Psychology, Norwegian University of Science and Technology, ³Gillberg Neuropsychiatry Centre, University of Gothenburg, ⁴P. Bechtereva Institute of the Human Brain, Russian Academy of Sciences, ⁵Department of Neuropsychology, Andrzej Frycz-Modrzewski Krakow University

EEG-methods are applied for extracting biomarkers of brain dysfunctions. The focus is on multi-channel event-related potentials (ERPs) recorded in a cued GO/NOGO task. Non-brain artifacts are corrected and ERPs are compared with the normative data. Examples relate to biomarkers for ADHD diagnosis and prediction of medication response.

Bioengineering

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Charissa Poon^1,2, Melina Mühlenpfordt^*3, Marieke Olsman^*3, Spiros Kotopoulis^4,5, Catharina de Lange Davies³, Kullervo Hynynen^1,2,6

¹Physical Sciences Platform, Sunnybrook Research Institute, ²Institute of Biomedical Engineering, University of Toronto, ³Department of Physics, Norwegian University of Science and Technology, ⁴Department of Clinical Medicine, University of Bergen, ⁵Exact Therapeutics AS, ⁶Department of Medical Biophysics, University of Toronto

This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability.

Bioengineering

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Charlotte Nawijn¹, Tim Segers^1,2, Guillaume Lajoinie¹, Ýrr Mørch³, Sigrid Berg^4,5,6, Sofie Snipstad^3,6,7, Catharina de Lange Davies⁷, Michel Versluis¹

¹Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, ²BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, ³Department of Biotechnology and Nanomedicine, SINTEF Industry, ⁴Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, ⁵Department of Health Research, SINTEF Digital, ⁶Cancer Clinic, St. Olav’s Hospital, ⁷Department of Physics, Norwegian University of Science and Technology

The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales.