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Organoid Culture from a Single Intestinal Stem Cell

DOI :

10.3791/200008-v

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2:05 min

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April 7th, 2023

April 7th, 2023

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784 Views
1Suntory Foundation for Life Sciences, Bioorganic Research Institute, 2Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS), Kyoto University, 3Faculty of Medicine, Osaka Medical and Pharmaceutical University

Tags

Organoid Culture
Single Intestinal Stem Cell
Erythropoietin producing Hepatocellular Receptor B2
FEB2 positive
Fluorescence activated Cell Sorting
FEB2 Expression
High
Medium
Low
Negative
Centrifugation
ECM Embedding
24 well Plate
Singlets
Microliters
Incubate
Carbon Dioxide
Polymerization
Culture Medium
Row associated Kinase
Rock Inhibitor
Inverted Microscope
Magnification
Viable Organoids
Spheroid Formation
Crypt Protrusion

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Organoid Culture from a Single Intestinal Stem Cell

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