Name: heterotopic heart transplantation in mice
Uploaded: 2007-07-19T00:00:00.000+00:00
Duration: 23 min 43 s
Description: Watch this Scientific Journal Video about Heterotopic Heart Transplantation in Mice at JoVE.com

捐助者的心脏的制备及收获： <ol><li style="text-align: left">该鼠标是与腹腔注射巴比妥和手术视野放在仰卧麻醉。 </li><li style="text-align: left">是一个长期的中线腹部切口。以暴露腹主动脉和下腔静脉，腹腔内容物用纱布缩回到左。 </li><li style="text-align: left"> 1毫升肝素（10u/ml）注入下腔静脉肝素。 </li><li style="text-align: left">开胸脊柱两侧的肋骨上存在分歧，前胸壁杠杆暴露心脏。 </li><li style="text-align: left">下腔静脉，上腔静脉和奇静脉静脉结扎6-0丝，分为优越的连字。 </li><li style="text-align: left">升主动脉和肺动脉的分离，分为远端尽可能远。 </li><li style="text-align: left">肺静脉结扎，分为远端的关系。 </li><li style="text-align: left">心脏灌注4 C O盐溶液。 </li><li style="text-align: left">心脏被保存在冰冷的生理盐水溶液。 </li></ol>收件人的制备及移植<ol><li style="text-align: left">该鼠标是与腹腔注射巴比妥和手术视野放在仰卧麻醉。 </li><li style="text-align: left">是一个长期的中线腹部切口。缩回腹腔内容外，用纱布腹部暴露腹主动脉和下腔静脉。 </li><li style="text-align: left">暴露腹主动脉和下腔静脉的分支，并用10-0缝线结扎。 </li><li style="text-align: left">主动脉和下腔静脉的近端和远端连字周围放置。 </li><li style="text-align: left"> venotomy是在用30号针头的收件人的下腔静脉。开幕式，然后再扩展到一个长度等于捐助的微型剪刀肺主动脉。 </li><li style="text-align: left"> aortotomy是在30号针头与腹主动脉的收件人。 </li><li style="text-align: left">打开腹主动脉和下腔静脉盐水灌溉。 </li><li style="text-align: left">捐赠者的心放在收件人的腹部左侧，并用纱布覆盖。 </li><li style="text-align: left">留缝线捐助的升主动脉与受体腹主动脉的近端和远端的顶点。 </li><li style="text-align: left">收件人的腹主动脉和捐助者的升主动脉的右侧吻合完成后继续缝合。 </li><li style="text-align: left">第一个捐赠者的捐赠者的肺动脉和收件人的下腔静脉吻合肺动脉近端顶点与收件人的下腔静脉远端心尖两呆缝线。 </li><li style="text-align: left">左墙的下腔静脉和捐赠者的肺动脉与下腔静脉内继续缝合关闭。 </li><li style="text-align: left">与近端先端留缝合后打结，下腔静脉和捐助的肺动脉的右墙以外的下腔静脉缝合关闭。 </li><li style="text-align: left">捐赠者的心交给收件人的腹部右侧。收件人的腹主动脉和捐赠者的升主动脉吻合的右墙封闭，继续缝合。 </li><li style="text-align: left">吻合后，远端结扎删除，检查吻合口出血。 </li><li style="text-align: left">如果有一点没有出血，吻合口近端结扎删除。捐赠者的心立即充满血液，心的颜色变成红色，并开始再次击败。 </li><li style="text-align: left">肠子都返回到腹部，这是连续用6-0缝线封闭。 </li><li style="text-align: left">收件人鼠标放在一个温暖的地方。一小时后，收件人的复苏。 </li></ol>

<ol>
	<li>Corry, R. o. b. e. r. t. . J., Winn, H. e. n. r. y. . J., Russell, P. a. u. l. . S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Primarily+vascularized+allografts+of+heart+in+mice.">Primarily vascularized allografts of heart in mice.</a> Transplantation. 16, 343-350 (1973).</li></ol>

结果 我们已经成功地在小鼠异位心脏移植1000余例，并取得90％以上的成功率在不同品系小鼠，包括野生型，转基因和基因敲除小鼠。 讨论 由于博士。科里和罗素介绍，1973年在小鼠异位心脏移植模型，该模型已被证明是一个特别有价值的研究免疫排斥反应和开发新的免疫策略。这种模式可以更广泛地使用，因为现在出现了大量的转基因和基因敲除小鼠研究排斥/宽容的?...

heterotopic heart transplantation in mice

小鼠异位心脏移植已被广泛使用，因为它是由博士介绍。科里和罗素在1973年。它特别适用于研究排斥反应和免疫反应，现在，新的转基因和基因敲除小鼠可宝贵的，已经开发和大量的免疫试剂。心脏移植模型是严格小于皮肤移植模型，虽然在技术上更具挑战性。我们已经开发出一种改性技术，并已完成超过1000对小鼠异位心脏移植成功的病例。升主动脉和腹主动脉吻合时，两呆缝线放置捐助小号升主动脉与受体腹主动脉的近端和远端的顶点，然后使用主动脉两侧11-0继续缝线缝合吻合。留缝线吻合更容易和11-0是一个理想的缝合的大小，以避免出血和血栓形成。 肺动脉和下腔静脉吻合时，两呆缝线捐助小号肺动脉近端顶点与收件人小号下腔静脉远端心尖。下腔静脉和捐助小号肺动脉左侧壁与下腔静脉内继续缝合封闭后，与近端顶点住宿结缝合下腔静脉的右侧壁和捐助小号肺动脉外下腔洞穴，用10-0缝线缝合继续封闭。这种方法更容易执行，因为只是一方的下腔静脉和10-0缝线吻合，是正确的大小，以避免出血和血栓形成。在这篇文章中，我们提供的技术，以补充视频的细节。

Watch this Scientific Journal Video about Heterotopic Heart Transplantation in Mice at JoVE.com

Heterotopic Heart Transplantation in Mice

小鼠异位心脏移植模型已被证明许多研究者排斥反应和免疫反应机制研究的重要方法。然而，所涉及的技术仍然充满挑战。通过修改标准的技术，我们已与超过1000移植成功。

小鼠异位心脏移植

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable ...

My name is Hun Li.I work at Dr.Kas Lab, the Department of Surgery, university of California, San Francisco. Today I will show you the procedures for the heterotopic heart transplantation in mice. This model is very important tools for organ transplantation field since it was first introduced by doctors Carrie and Russell in 1973.This is extremely valuable model for studying new immunosuppressive drugs and mechanisms of re rejection. Higher technical difficulties have been limited. This model by suppress used, I have modified some steps.Make this procedure easier. I'm sure you they'll be hyper for you when you do this model.Okay? I have just shown you the procedure for heterotopic heart transplantation in mice.The mo, the most important steps in this procedure is suitable density of sutures. This should not be too low or too tight, and osmosis should be finished in 30 minutes. This transplantation model is extremely useful for study of immunological responses after organ transplantation and ing for new immunosuppressive drugs.I hope now you'll be able to carry out these procedures in your own laboratories. Thank you and.

heterotopic-heart-transplantation-in-mice

Research

JoVE Journal

Biology

29.4K 次观看.  University of California, San Francisco - UCSF. My name is Hun Li.I work at Dr.Kas Lab, the Department of Surgery, university of California, San Francisco. Today I will show you the procedures for the heterotopic heart transplantation in mice. This model is very important tools for organ transplantation field since it was first introduced by doctors Carrie and Russell in 1973.This is extremely valuable model for studying new immunosuppressive drugs and mechanisms of re rejection. Higher technical difficulties have been limited. This...

视频: Heterotopic Heart Transplantation in Mice

A Modified Method for Heterotopic Mouse Heart Transplantion

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents available. A difficulty is presented due to the small size of the animal and the considerable technical challenges of the microsurgery involved in heart transplantation. In particular, a high rate of technical failure early after transplantation may result from recipient death and post-operative complications such as hind limb paralysis or a non-beating heart. Here, the complete technique for heterotopic mouse heart transplantation is demonstrated, involving harvesting the donor heart and its subsequent implantation into a recipient mouse. The donor heart is harvested immediately following in situ perfusion with cold heparinized saline and transection of the ascending aorta and pulmonary artery. The recipient operation involves preparation of the abdominal aorta and inferior vena cava (IVC), followed by end-to-side anastomosis of the donor aorta with the recipient aorta using a single running 10-0 microsuture and a similar anastomosis of the donor pulmonary artery with the recipient IVC. Following the operation the animal is injected with 0.6 ml normal saline subcutaneously and allowed to recover on a 37&#160;&#176;C heating pad. The results from 227 mouse heart transplants are summarized with a success rate at 48 hr of 86.8%. Of the 13.2% failures within 48 hr, 5 (2.2%) experienced hind limb paralysis, 10 (4.4%) had a non-beating heart due to graft ischemic injury and/or thrombosis, while 15 (6.6%) died within 48 hr.

A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.

对于异位小鼠心脏移植技术的一种改进方法

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents ...

科研

医学

Murine Heterotopic Heart Transplant Technique

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique was developed and reported in 2001 by Niimi. Described here are the techniques that have evolved over more than a decade in the hands of three surgeons (Plenter, Grazia, Pietra) in our center. These techniques are now being passed on to a younger generation of surgeons and researchers.
Based largely on the Niimi experience, the procedures used have evolved in the fine details - details which we will endeavor to relate here in such a way that others may be able to use this very useful model. Like Niimi, we have found that a video aid to learning is a priceless resource for the beginner.

The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.

小鼠异位心脏移植技术

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed results. However, the cellular and molecular mechanisms of the neotissue development, valve thickening, and stenosis development are not researched extensively. To answer the above questions, we developed a murine heterotopic heart valve transplantation model. A heart valve was harvested from a valve donor mouse and transplanted to a heart donor mouse. The heart with a new valve was transplanted heterotopically to a recipient mouse. The transplanted heart showed its own heartbeat, independent of the recipient&#8217;s heartbeat. The blood flow was quantified using a high frequency ultrasound system with a pulsed wave Doppler. The flow through the implanted pulmonary valve showed forward flow with minimal regurgitation and the peak flow was close to 100 mm/sec. This murine model of heart valve transplantation is highly versatile, so it can be modified and adapted to provide different hemodynamic environments and/or can be used with various transgenic mice to study neotissue development in a tissue engineered heart valve.

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

肺动脉瓣使用异位心脏移植小鼠模型移植

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Heterotopic Cervical Heart Transplantation in Mice

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient&#8217;s common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.

The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.

颈椎异位心脏移植小鼠

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats

Heterotopic heart transplantation in rats has been a commonly used model for diverse immunological studies for more than 50 years. Several modifications have been reported since the first description in 1964. After 30 years of performing heterotopic heart transplantation in rats, we have developed a simplified surgical approach, which can be easily taught and performed without further surgical training or background.
After dissection of the ascending aorta and the pulmonary artery and ligation of superior and inferior caval and pulmonary veins, the donor heart is harvested and subsequently perfused with ice-cold saline solution supplemented with heparin. After clamping and incising the recipient abdominal vessels, the donor ascending aorta and pulmonary artery are anastomosed to the recipient abdominal aorta and inferior vena cava, respectively, using continuous running sutures.
Depending on different donor-recipient combinations, this model allows analyses of either acute or chronic rejection of allografts. The immunological significance of this model is further enhanced by a novel approach of in-ear injection of vital cardiac muscle cells and subsequent analysis of draining cervical lymphatic tissue.

We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

大鼠异位心脏和心肌细胞移植的免疫模型

Heterotopic heart transplantation in rats has been a commonly used model for diverse immunological studies for more than 50 years. Several modifications ...

免疫与感染

Optimization of the Cuff Technique for Murine Heart Transplantation

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps. First, we used the inner tube technique to insert a temporary inner tube into the external jugular vein and carotid artery blood vessel to facilitate eversion of the vessel over the cuff. Second, we performed complete heterotopic cardiac transplantation through the collaboration of two experienced surgeons. These modifications effectively reduced the operation time to 25 minutes, with a success rate of 95%. In this report, we describe these procedures in detail and provide a supplemental video. We believe that this report on the improved cuff technique will offer practical guidance for murine heterotopic heart transplantation and will enhance the utility of this mouse model for basic research.

We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time.

穆林心脏移植袖口技术的优化

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after transplantation, and the impact of immunomodulatory therapies on graft rejection. Heterotopic cervical heart transplantation in mice was first described in 1991 using sutured anastomoses and subsequently modified to include cuff techniques. This modification allowed for improved success rates, and since then, there have been multiple reports that have proposed further technical improvements. However, translation into more widespread utilization remains limited due to the technical difficulty associated with graft anastomoses, which requires precision to achieve adequate length and caliber of the cuffs to avoid vascular anastomotic twisting or excessive tension, which can result in damage to the graft. The present protocol describes a modified technique for performing heterotopic cervical cardiac transplantation in mice which involves cuff placement on the recipient's common carotid artery and the donor's pulmonary artery in alignment with the direction of the blood flow.

Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

利用血管袖带的小鼠异位宫颈心脏移植

Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after ...

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

The surgical technique of heterotopic abdominal heart transplantation in mice is a standard model for research in transplantation immunology. Here, the established technique for a modified blood circuit reconstruction in a heterotopic abdominal heart transplantation model is presented. This method uses the intrathoracic inferior vena cava (IIVC) instead of the pulmonary artery of the donor heart for the anastomosis to the inferior vena cava of the recipient. It is facilitating and improving success rates for abdominal heart transplantation in mice.

A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

腹部小鼠心脏移植模型中的血液回路重建

The surgical technique of heterotopic abdominal heart transplantation in mice is a standard model for research in transplantation immunology. Here, the ...

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for investigating the mechanism of cardiac allograft rejection in preclinical studies because of their high homology with human genes. This understanding would help develop unique approaches to improving patients&#39; long-term survival treated with cardiac allografts. In a mouse model, abdominal donor heart implantation is commonly performed with an end-to-end&#160;anastomosis to the recipient&#39;s aorta and inferior vena cava using stitches. In this model, the donor&#39;s heart is implanted by end-to-end anastomosis to the recipient&#39;s carotid artery and jugular vein by the modified-Cuff technique. The transplantation surgery is performed without stitching and thus may increase the survival of the recipient since there is no interference with the blood supply and venous reflux of the lower body. This mouse model would help investigate the mechanisms underlying the immunological and pathological (acute/chronic) rejection of cardiac allografts.

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

一种改进的袖带技术用于小鼠颈椎异位心脏移植模型

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for ...