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09:43 min
March 26th, 2013
DOI :
10.3791/50325-v
Chapters
0:05
Title
1:10
Chip Fabrication
4:33
Solid Phase Extraction Method
6:43
RT-PCR
8:06
PCR Products Detection
8:38
Results: The Microfluidic Assay Flow
9:24
Conclusion
Summary
An integrated microfluidic thermoplastic chip has been developed for use as a molecular diagnostic. The chip performs nucleic acid extraction, reverse transcriptase, and PCR. Methods for fabricating and running the chip are described.
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