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JoVE Journal

Neuroscience

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Preparation of Neuronal Co-cultures with Single Cell Precision

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09:06 min

May 20th, 2014

May 20th, 2014

13,608 Views

0:05

Title

2:04

Microfluidic Device Replication in PDMS

3:26

Device Assembly

4:40

Water Masking for In situ Biomaterial Patterning by Plasma Stenciling

6:39

Microfluidic Single Neuron Arraying and Culture

7:49

Selective Fluidic Treatment

8:37

Conclusion

Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs.

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