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JoVE Journal

Chemistry

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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

20.3K Views

13:00 min

April 4th, 2014

April 4th, 2014

20,286 Views

0:05

Title

2:01

Preparing Samples and Performing the Experiment

6:47

Data Analysis

9:24

Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry

12:29

Conclusion

Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.

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