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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

DOI :

10.3791/52772-v

June 10th, 2015

June 10th, 2015

11,807 Views

1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

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HeLa Based Cell Free Expression Systems

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