Genomic DNA Preparation Using a Modified Sodium Hydroxide Method
4:40
Design Sex-specific PCR Primers and Genomic DNA PCR
7:34
Results: Porcine Embryo Sex Determination
8:54
Conclusion
副本
The overall goal of this procedure is to accurately identify the sex of day 30 pig embryos, using phenol free DNA obtained from each embryo. And two pairs of sex-specific primers for a PCR reaction. This method has the potential to answer various
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This protocol describes an accurate, inexpensive, rapid and non-toxic method to determine the sex of Day 30 porcine embryo using PCR method after grinding an embryo into powder without phenol chloroform extraction and DNA column purification.