Results: Characteristics of Primary Microglial Cultures
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Conclusion
副本
The overall goal of CD11b magnetic microglia isolation is to achieve a high purity end yield of postnatal microglia in a relatively short time period for the purposes of versatile in vitro experimentation. This method can help answer key questions
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Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.