March 9th, 2018
•Efforts to understand microglial function in detail have been hindered by the lack of microglial culture models that recapitulate the properties of mature in vivo microglia. This protocol describes an isolation and culture approach designed to maintain robust survival of highly ramified mature rat microglia under defined-medium conditions.
Tags
Related Videos
Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
Dynamic Quantitative Sensory Testing to Characterize Central Pain Processing
Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments