Primary Mouse Neonatal Skin Keratinocyte (KC) Isolation and Culture
5:06
Primary Mouse Adult Tail Skin KC Isolation
6:49
In Vitro High Calcium-induced KC Differentiation, UVB-mediated KC Death, and KC TNFα Release Quantification
7:55
Results: Representative KC Imaging and Analysis
9:58
Conclusion
副本
The overall goal of this procedure is to isolate primary mouse keratinocytes from neonatal or adult mouse skin from their in-vitro differentiation or to study their innate immune responses to UVb exposure. This method can help answer key questions
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Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.