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JoVE Journal

Immunology and Infection

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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

7.0K Views

08:38 min

October 30th, 2017

October 30th, 2017

7,006 Views

0:05

Title

0:51

In Vitro Chemotaxis

2:56

Murine Lymphocyte Adoptive Transfer

5:17

Collection and Analysis

6:28

Results: Representative In Vitro and In Vivo Migration Analyses

7:44

Conclusion

副本

The overall goals of these assays are to quantify monocyte chemotaxis in vitro in real time and to track lymphocyte migration in vivo. The in vitro method can help answer key questions in the field of immunology, such as what are the effects of se

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Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

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