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JoVE Journal

Developmental Biology

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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

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07:18 min

April 19th, 2018

April 19th, 2018

20,217 Views

0:05

Title

0:41

Cryo-embedding of the Tibialis Anterior Muscle

1:46

Cryostat Sectioning

2:33

Immunofluorescence and Staining

5:27

Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining

6:40

Conclusion

副本

The overall goal of this protocol is to identify adult skeletal muscle satellite cells via immunofluorescence. This method can answer key questions related to the number and distribution of muscle stem cells in both physiological as well as pathol

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The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

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