Culturing of Mycobacterium marinum and Preparation of Bacterial Solution for Infecting Adult Zebrafish
2:10
Experimental M. marinum Infection with Intraperitoneal Injection
3:32
Collection of Internal Organs
4:36
Results: Mycobacterium marinum Infection Modeling
6:02
Conclusion
副本
This method can help to answer key questions in the field of tuberculosis such as what is the role of adaptive immune responses and the pathogenesis of this multifactorial disease. The main advantage of this nucleic acid base method is that both b
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Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.
