Immunomagnetic Separation (IMS) and Multiple Displacement Amplification (MDA)
3:37
Real-time PCR and Library Preparation for Quasimetagenomic Sequencing
4:52
Results: RT-PCR Amplifies DNA from Two Brands of Salmonella Contaminated Chicken Breast
5:19
Conclusion
副本
This method can be used to detect Salmonella from food samples and identify the pathogen to the strain level through genetic fingerprinting. The main advantage of this technique is that it combines Salmonella detection and subtyping into a single
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Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples.
