Results: Representative Islet Cell Responses to Various Stimuli
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Conclusion
副本
The protocol allows rapid isolated function of small subpopulations of cells within pancreatic islets of Langerhans. The advantages of this technique include the real-time nature and high statistical power, and hence high reproducibility, of the a
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Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.