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Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

DOI :

10.3791/60032-v

September 18th, 2019

September 18th, 2019

9,355 Views

1College of Pharmacy, Duksung Innovative Drug Center, Duksung Women's University, 2Korea Drug Development Platform using Radio-isotope, Korea Institute of Radiological & Medical Sciences

Three-dimensional co-culture spheroid angiogenesis assay system is designed to mimic the physiologic angiogenesis. Co-culture spheroids are formed by two human vascular cell precursors, ECFCs and MSCs, and embedded in collagen gel. The new system is effective for evaluating angiogenic modulators, and provides more relevant information to the in vivo study.

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Keywords 3D Co culture Spheroid

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