Cell-Cell Fusion Immunofluorescent Staining and Imaging
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Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging
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Conclusion
副本
This method allows cellular structure and function to be perturbed through the fusion of two different cell types to address questions such as how do cells respond to changes in organelle number. By imaging fluorescently tagged proteins within fus
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The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.