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Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

DOI :

10.3791/63336-v

February 10th, 2022

February 10th, 2022

4,356 Views

1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III

Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.

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Isolation

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