Muscle Dissection, Homogenization, and Mitochondrial Isolation
4:35
Preparation of the Microplate-Based Respirometric Assays
6:17
Results: Mitochondria Isolation from Mouse Skeletal Muscle
7:32
Conclusion
필기록
This word describes respiration analysis in processing mitochondria from mouse skeletal muscle. This protocol considerably reduce purification time and allows multiple samples to be processed simultaneously. Mitochondria from animals at any eight
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Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.