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JoVE Journal

Biochemistry

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The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples


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07:27 min

June 29th, 2022

June 29th, 2022

2,173 Views

0:04

Introduction

0:33

Preparation Steps for the Peel‐Blot Technique

1:57

Placing the Carbon Film onto the Grid

3:07

Separation of Multi-Layered 2D Crystals into Single Layer

4:55

Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper

6:42

Conclusion

副本

The peel-blot technique allows for the separation of multi-layered and concentrated biological cryo-EM samples into single layers to reduce thickness, increase concentration, and facilitate image processing. Sample concentrations can be increased

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The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image processing.

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