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The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

2.2K Views

07:27 min

June 29th, 2022

DOI :

10.3791/64099-v

June 29th, 2022


Transcript

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Peel Blot Technique

Chapters in this video

0:04

Introduction

0:33

Preparation Steps for the Peel‐Blot Technique

1:57

Placing the Carbon Film onto the Grid

3:07

Separation of Multi-Layered 2D Crystals into Single Layer

4:55

Results: 2D Crystals of Human Leukotriene C4 Synthase and Liposomes Subjected to the Peel‐Blot Method, the Peel-Blot Footprint, and Blotting an EM Grid Prepared by Back-Injection on a Submicron Filter Paper

6:42

Conclusion

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