基于转录组学的经济高效的药物筛选

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06:40 min

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February 23rd, 2024

DOI :

10.3791/65930-v

February 23rd, 2024

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Jacqueline Leidner¹^,², Heidi Theis³, Michael Kraut³, Alice Ragogna¹, Marc Beyer¹^,³^,⁴, Joachim Schultze¹^,²^,³, Jonas Schulte-Schrepping¹^,², Caterina Carraro¹^,², Lorenzo Bonaguro¹^,²

¹Systems Medicine, German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute, University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE) eV.

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0:00

Introduction

1:08

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing

3:01

cDNA Library Preparation from the Drug Treated‐Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing