Pooled CRISPR sgRNA Lentiviral Library Plasmid Amplification
2:24
Large-scale CRISPR sgRNA Library Lentivirus Production
3:47
Primary Screen Infection, Selection, and Cell Passaging
5:41
CRISPR Sample Preparation and Sequencing
6:43
Results: Genome-scale CRISPR Screening
7:54
Conclusion
副本
CRISPR screened drop-out screens provide researchers with a simple, efficient, and inexpensive method to interrogate chain function on the genome-wide level. The advantage of CRISPR is its pliability to edit any gene by simply changing the guide s
登录或开始您的免费试用以访问此内容
CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.