我们感谢布莱根妇女医院和哈佛医学院的 Mel Feany 博士对照和表达 果蝇 系的 α-突触核蛋白的善意礼物。我们感谢以下对 Olsen 博士的资助来源:NINDS K08、美国帕金森病协会 George C. Cotzias 奖学金、国防部帕金森病早期研究者奖。

用于该测定的果蝇:对照:nSyb-QF2、nSyb-Gal4/+;α-突触核蛋白果蝇:nSyb-QF2、nSyb-Gal4、QUAS-α-突触核蛋白/+;关闭后 1 天和 10 天;雄性和雌性（交配和未交配）苍蝇（见 材料表）。
1.准备染色的苍蝇食品
<ol><li>将蓝色软凝胶糊食用色素（见 材料表）与蒸馏水以 1:1 的比例 （v/v） 混合。 注意:使用仅含有不可吸收染料的商业食用色素，因为一些蓝色染料已被证明具有神经保护作用21.</li>
	<li>以 10-15 秒的间隔用微波炉加热由标准玉米面 - 琼脂（见 材料表）组成的苍蝇食品小瓶，直到食物融化成液体或浆液。不要让食物沸腾。</li>
	<li>将染料混合物添加到每瓶食物中，以获得小瓶之间的均匀着色。混合在染料混合物中，直到食物呈均匀的蓝色（图1）。加入一定量的蓝色染料混合物，使食物的颜色饱和，并且小瓶之间的颜色没有变化。 注意: 此步骤必须在微波炉加热后立即完成，因为食物会很快凝固。如果食物凝固，请再次用微波炉加热小瓶。</li>
	<li>将染色食品小瓶风干直至凝固。干燥时将薄纸巾放在小瓶开口上，以防止任何流浪苍蝇落在小瓶中。</li>
	<li>食物冷却凝固后，将用于测试的苍蝇转移到蓝色食物中。 注意:对于窄瓶（25 毫米），建议在每个小瓶中添加 9-14 只苍蝇。对于宽小瓶（28.5 mm），建议在每个小瓶中加入10-20只苍蝇。苍蝇不按性别或交配经验分开，包括雄性和雌性苍蝇的混合种群，包括交配和未交配的雌性。</li>
	<li>将小瓶与苍蝇一起在培养箱中25°C孵育过夜。</li>
</ol>2. 对苍蝇进行成像
注意:此步骤是可选的（图 2）。
<ol><li>第二天早上（时间0），用二氧化碳麻醉代表苍蝇60秒。放置苍蝇，使腹侧朝上。</li>
	<li>使用相机（见 材料表），每小时捕捉苍蝇的图像，以可视化消化系统中剩余的蓝色食物量。 注意:这些苍蝇不应用于测定的定量部分（步骤3），因为麻醉可能会影响结果。</li>
	<li>每小时对新苍蝇进行麻醉和成像。</li>
</ol>3.进行便秘测定
<ol><li>将剩余的（未麻醉的）苍蝇转移到装有标准 果蝇 食物的小瓶中。对每个小瓶进行编号。</li>
	<li>将苍蝇留在25°C的培养箱中60分钟。</li>
	<li>60分钟后，将苍蝇转移到装有标准 果蝇 食物的新小瓶中。从第一组样品瓶开始数据记录。</li>
	<li>在小瓶的长度上画一条虚线，以标记开始计数的点。</li>
	<li>手动计算每个小瓶壁上小圆点的数量。不要计算在食物或小瓶塞上发现的点。</li>
	<li>首先计算每个小瓶壁上的蓝点数量。</li>
	<li>计算每个小瓶壁上不透明的无色点的数量。 注意:每个点是苍蝇粪便，它是尿液和粪便的组合 22.有时，苍蝇可能会走过粪便并留下痕迹，在这种情况下，只计算原始点，而不是痕迹中的每个标记。</li>
	<li>记录每个小瓶的蓝点与点总数的比率。 注意:点总数是小瓶壁上的蓝点数与同一小瓶壁上的无色点数相加。这将用于计算每小时蓝色粪便的百分比。</li>
	<li>重复步骤 3.2-3.8 七次，每小时收集一次数据，总共 8 小时。</li>
</ol>4. 数据分析
<ol><li>绘制每种情况下每小时蓝色粪便的百分比。</li>
	<li>比较条件之间的数据。 注意:统计显著性可以通过 3 种方式确定:通过比较条件之间的各个时间点、测量线随时间变化的斜率或比较曲线下的面积。</li>
</ol>

果蝇中的便秘测定

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作者没有什么可透露的。

该协议中有几个步骤将有助于成功完成测定。首先，重要的是要确保每个小瓶的每轮之间的时间间隔在整个实验过程中保持一致。用数字标记小瓶将有助于避免冗长的基因型描述，从而节省时间。此外，在整个实验过程中，粪便计数方法22 保持一致至关重要。虽然在食物和小瓶塞上可以看到蓝色粪便，但无色粪便则不然。因此，不要计算食物或小瓶塞上的蓝点。
在行为测定中，由于苍蝇行为的波动或影响测定的未知变量，结果总是存在不一致的可能性。我们建议在所有实验中使用相同的 果蝇 培养基、相同的食用色素和相同品牌的小瓶。有趣的是，在几项试验中，观察到苍蝇在下午早些时候排便的频率较低，这可能是由于苍蝇的昼夜节律23.然而，这种行为在对照果蝇和表达α-突触核蛋白的果蝇中是一致的，因此不应引起关注。
如果苍蝇在测定开始时没有排泄蓝色粪便，则可能使用的染料色素沉着不足。在这种情况下，可以相应地增加染料与蒸馏水的比例。也有可能当苍蝇的消化道中只剩下少量蓝色食物时，可能很难确定粪便是淡蓝色还是无色。发生这种情况时，在小瓶后面放一张白纸将有助于确定粪便点的颜色。即使粪便是很浅的蓝色，最好将其记录为蓝色粪便而不是无色粪便。
该检测的一个局限性是它需要手动计数粪便斑点。为了提高高通量筛选的潜力，该方案将来可能会被修改，以允许自动定量多孔板中单个果蝇产生的蓝色粪便斑点。另一个局限性是，尽管 α-突触核蛋白模型有可能发展成为 PD 的前驱模型，但尚未确定存在便秘而没有神经退行性变的最佳时间点。
总之，这种方法提供了一种简单、直接的方法来模拟果蝇模型中的便秘，便秘是一种未被充分研究的非运动性 PD 症状。

帕金森病 （PD） 是一种进行性神经退行性疾病，临床特征是存在运动迟缓、强直和震颤等运动症状，导致显着的发病率1。在病理学上，PD的定义是黑质中多巴胺能神经元的丢失和α-突触核蛋白的错误折叠，导致路易体和路易神经突的形成。帕金森病的发病机制仍然知之甚少，可能是由遗传和环境因素的复杂相互作用引起的 2,3。目前，尚无改善疾病的疗法，部分原因可能是诊断时病理学已处于晚期。研究表明，黑质中超过 60% 的多巴胺能神经元已经因运动症状的发作而丢失，这强调了探索早期疾病检测的潜在生物标志物的必要性4.便秘就是这样一种临床生物标志物，这在帕金森病患者中很常见 5,6，并且可能在运动症状出现之前数年甚至数十年。
尽管 PD 的临床定义基于运动症状，但便秘是几种非运动症状之一，这些症状对症治疗更具挑战性，并导致患者生活质量的显着损害7.代表大脑和肠神经系统之间双向交流的肠脑轴的改变与帕金森病的发病机制有关。在帕金森病患者胃肠道的组织样本中发现了 α-突触核蛋白聚集体8，动物模型表明肠道神经系统中的 α-突触核蛋白聚集体以朊病毒样方式扩散到中枢神经系统9。此外，帕金森病患者在肠道微生物组中表现出异常10，并可能经历过度的肠道炎症11。帕金森病的便秘研究不足，很少有报道的苍蝇 12,13 或啮齿动物14,15 中帕金森氏症相关便秘的模型。
该测定采用果蝇 PD 模型，其中果蝇在泛神经元驱动因子 n-突触反射蛋白的控制下表达人类 α-突触核蛋白基因。这些果蝇表现出 PD 的所有标志性特征，包括 α-突触核蛋白聚集、运动功能障碍和与年龄相关的神经退行性变，导致多巴胺能神经元的丢失16,17。以前的研究引入了测量果蝇粪便输出量以评估肠道功能障碍，量化果蝇粪便并比较各种遗传系的排泄物量，以揭示消化系统的功能差异18,19,20。在这里，我们演示了使用表达人α-突触核蛋白的果蝇的便秘测定。这个简单而有价值的工具可以研究帕金森病的重要非运动症状。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1400 g sucrose</td>
			<td>MP Biomedicals</td>
			<td>904713</td>
			<td></td>
		</tr>
		<tr>
			<td>1800 g dextrose</td>
			<td>MP Biomedicals</td>
			<td>901521</td>
			<td></td>
		</tr>
		<tr>
			<td>2884 g yeast</td>
			<td>MP Biomedicals</td>
			<td>903312</td>
			<td></td>
		</tr>
		<tr>
			<td>428 g agar</td>
			<td>Fisher Scientific</td>
			<td>10253156</td>
			<td></td>
		</tr>
		<tr>
			<td>4600 mL molasses</td>
			<td>Grandma&#39;s Molasses</td>
			<td>7971942</td>
			<td></td>
		</tr>
		<tr>
			<td>68 L water</td>
			<td>N/A</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>680 mL tegosept mix (1200 g tegosept in 6 L ethanol)</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>6864 g cornmeal</td>
			<td>Pearl Milling</td>
			<td>125045</td>
			<td></td>
		</tr>
		<tr>
			<td>800 mL acid mix (83 mL phosphoric acid in 1 L water + 836 mL propionic acid in 1 L water)</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>cellSens Standard software</td>
			<td>Olympus</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>Pharmco-Aper</td>
			<td>111ACS200</td>
			<td></td>
		</tr>
		<tr>
			<td>Flugs for wide plastic vials</td>
			<td>Genesee Scientific</td>
			<td>49-101</td>
			<td></td>
		</tr>
		<tr>
			<td>Flystuff wide Drosophila vials, polystyrene</td>
			<td>Genesee Scientific</td>
			<td>32-117</td>
			<td></td>
		</tr>
		<tr>
			<td>Graphpad Prism</td>
			<td>GraphPad</td>
			<td>N/A</td>
			<td>Version 9.5.1</td>
		</tr>
		<tr>
			<td>Olympus DP23 camera</td>
			<td>Olympus</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Olympus SZX12 Stereo Microscope</td>
			<td>Olympus</td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>Phosphoric Acid</td>
			<td>Fisher Scientific</td>
			<td>S25470A</td>
			<td></td>
		</tr>
		<tr>
			<td>Propionic Acid</td>
			<td>Fisher Scientific</td>
			<td>A258 - 500&#160;</td>
			<td></td>
		</tr>
		<tr>
			<td>Soft gel paste food color, Royal blue</td>
			<td>AmeriColor</td>
			<td>202</td>
			<td></td>
		</tr>
		<tr>
			<td>Tegosept</td>
			<td>Apex</td>
			<td>20-258</td>
			<td></td>
		</tr>
		<tr>
			<td>Drosophila Stocks</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>nSyb-QF2, nSyb-Gal4</td>
			<td>All lines provided by Dr. Mel Feany</td>
			<td>N/A</td>
			<td>Lines are available directly from Dr. Feany&#160;</td>
		</tr>
		<tr>
			<td>nSyb-QF2, nSyb-Gal4, QUAS-alpha synuclein</td>
			<td></td>
			<td>N/A</td>
			<td></td>
		</tr>
		<tr>
			<td>w1118</td>
			<td></td>
			<td>N/A</td>
			<td></td>
		</tr>
	</tbody>
</table>

measuring constipation in a drosophila model of parkinson's disease

帕金森病 （PD） 的非运动症状很常见，难以治疗，并且会严重损害生活质量。一种普遍的非运动症状是便秘，它可能比诊断为帕金森病早数年甚至数十年。便秘在帕金森病的动物模型中尚未得到充分探索，并且缺乏特异性治疗。该测定利用了 PD 的 果蝇 模型，其中人 α-突触核蛋白在泛神经元驱动下表达。表达α-突触核蛋白的果蝇具有帕金森病的标志性特征:多巴胺能神经元的丧失、运动障碍和α-突触核蛋白包涵体。
该协议概述了一种研究这些苍蝇便秘的方法。将苍蝇放在带有蓝色添加剂的苍蝇食物上过夜，然后在第二天转移到标准食物中。随后将它们移至装有标准苍蝇食品的新小瓶中，每小时持续 8 小时。在每次转移之前，计算蓝色粪便斑点与小瓶壁上总粪便斑点的百分比。缺乏α-突触核蛋白的对照果蝇在表达α-突触核蛋白的果蝇之前数小时排出所有蓝色染料。此外，蓝色食物从肠道的通过可以通过简单的摄影来监测。该测定的简单性使其能够用于正向遗传或化学筛选，以鉴定 果蝇便秘的修饰剂。

因为果蝇的腹部是透明的，所以在活的麻醉果蝇中，可以在肠道内看到蓝色食物。可以通过在不同时间点拍摄苍蝇的图像来评估肠道运输的定性差异。在对照果蝇中，蓝色食物会迅速被排出，而在α-突触核蛋白果蝇中，蓝色食物在肠道中停留长达8小时（图2）。
α-突触核蛋白果蝇表现出与年龄相关的神经变性，在闭合后 10 天形成健壮的表型10。在这个时间点，通过比较条件之间的各个时间点，通过测量线随时间变化的斜率或比较曲线下的面积，可以看到与对照果蝇相比肠道运输时间的差异（图3）。在较早的时间点，即闭合后第1天，在对照果蝇和α-突触核蛋白果蝇之间的肠道运输中没有观察到差异，此时运动功能障碍和神经退行性变也尚未存在（图4）。
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/65966/65966fig1v2.jpg"> 图 1:蓝色食品瓶和粪便斑点。 （A） 所有小瓶都含有蓝色食物。此时，苍蝇刚刚被引入蓝色食物，看不到蓝色粪便。（B） 将 w1118 苍蝇转移到新鲜食物中 1 小时后的小瓶，然后取出。左边的插图在（C）中放大，右边的插图在（D）中放大。（C） 由于存在苍蝇食物而被排除在分析之外的区域。（D） 粪便斑点的放大视图，表示蓝色斑点（黑色箭头）和非蓝色斑点（黄色箭头）。 <a href="https://www.jove.com/files/ftp_upload/65966/65966fig1v2large.jpg" target="_blank">请点击这里查看此图的较大版本.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/65966/65966fig02.jpg"> 图2:染色食品肠道运输随时间变化的可视化。 对照果蝇和在神经元中表达人类α-突触核蛋白的果蝇在染色的食物上过夜，然后在第二天转移到标准培养基中。苍蝇每小时被连续转移到新的标准苍蝇食物中。图中显示了时间零和转印后 2、4、6 和 8 小时的代表性图像。对照组和α-突触核蛋白果蝇在零点时在肠道中显示出类似的蓝色食物斑块。在随后的时间点，对照果蝇的蓝色食物比α-突触核蛋白果蝇少，并且在对照果蝇的8小时时间点，所有蓝色食物都消失了。各蝇系的基因型如下:对照（nSyb-QF2、nSyb-Gal4/+）;α-突触核蛋白苍蝇（nSyb-QF2、nSyb-Gal4、QUAS-α-突触核蛋白/+）。雌性苍蝇用于成像，因为它们的体型较大，更容易可视化腹部。 <a href="https://www.jove.com/files/ftp_upload/65966/65966fig2v2large.jpg" target="_blank">请点击这里查看此图的较大版本.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/65966/65966fig3v2.jpg"> 图 3:苍蝇闭合后第 10 天的便秘测定。 对照苍蝇和在神经元中表达人类α-突触核蛋白的果蝇在染成蓝色的食物上过夜，然后在第二天转移到标准苍蝇食物中。苍蝇每小时连续转移到新的标准培养基上。（A） 每小时蓝色粪便的百分比。误差线表示标准偏差。N = 每个基因型 6 瓶;每个小瓶含有 9-14 只苍蝇。所有统计分析均在Graphpad Prism中进行（参见 材料表）。使用双因素方差分析计算每个时间点的统计显着性。使用线性回归分析计算线的斜率和斜率之间的差值。（B） 计算每种基因型的曲线下面积。误差线表示标准偏差。各蝇系的基因型如下:对照（nSyb-QF2、nSyb-Gal4/+）;α-突触核蛋白苍蝇（nSyb-QF2、nSyb-Gal4、QUAS-α-突触核蛋白/+）。雄性和雌性苍蝇的数量大致相等。 <a href="https://www.jove.com/files/ftp_upload/65966/65966fig3v2large.jpg" target="_blank">请点击这里查看此图的较大版本.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/65966/65966fig4v2.jpg"> 图 4:苍蝇闭合后第 1 天的便秘测定。 对照果蝇和在神经元中表达人类α-突触核蛋白的果蝇在染色的食物上过夜，然后在第二天转移到标准培养基中。苍蝇每小时被连续转移到新的标准苍蝇食物中。（A） 每小时蓝色粪便的百分比。误差线表示标准偏差。N = 每种基因型至少 5 瓶;每个小瓶含有 9-14 只苍蝇。所有统计分析均在Graphpad Prism中进行。使用双因素方差分析计算每个时间点的统计显着性。使用线性回归分析计算线的斜率和斜率之间的差值。（B） 计算每种基因型的曲线下面积。误差线表示标准偏差。各蝇系的基因型如下:对照（nSyb-QF2、nSyb-Gal4/+）;α-突触核蛋白苍蝇（nSyb-QF2、nSyb-Gal4、QUAS-α-突触核蛋白/+）。雄性和雌性苍蝇的数量大致相等。 <a href="https://www.jove.com/files/ftp_upload/65966/65966fig4v2large.jpg" target="_blank">请点击这里查看此图的较大版本.</a>

Watch this Scientific Journal Video about Exploring Non-Motor Symptoms in Parkinson's Disease at JoVE.com

作者聚焦:探索帕金森病的非运动症状 

该协议提出了一种用于在基于α-突触核蛋白的帕金森病 果蝇 模型中模拟便秘的测定方法。

在帕金森病的 果蝇 模型中测量便秘

Non-motor symptoms in Parkinson's disease (PD) are common, difficult to treat, and significantly impair quality of life. One prevalent non-motor symptom ...

帕金森病的定义是运动迟缓、震颤和僵硬等运动症状。但许多帕金森病患者患有非运动症状，这些症状更难治疗，并导致更大的生活质量损害。这些尚未在该疾病的动物模型中得到很好的研究，这限制了我们为它们寻找新疗法的能力。该协议简单且价格低廉，但可产生可重复的结果。它易于学习，不需要专门的设备。通过这种简单的检测，我们可以评估患有神经退行性变的果蝇的便秘。未来，我们希望开发一种模型，其中苍蝇在神经退行性变发作之前会出现便秘。这将使我们能够更好地了解帕金森病的发病机制。

measuring-constipation-in-a-drosophila-model-of-parkinson-s-disease

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Measuring Constipation in a Drosophila Model of Parkinson's Disease

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Drugs Affecting Gastrointestinal Motility

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1.3K 次观看.  University of Pittsburgh. 帕金森病的定义是运动迟缓、震颤和僵硬等运动症状。但许多帕金森病患者患有非运动症状，这些症状更难治疗，并导致更大的生活质量损害。这些尚未在该疾病的动物模型中得到很好的研究，这限制了我们为它们寻找新疗法的能力。该协议简单且价格低廉，但可产生可重复的结果。它易于学习，不需要专门的设备。通过这种简单的检测，我们可以评估患有神经退行性?...

视频: 作者聚焦:探索帕金森病的非运动症状 

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson's Disease Model of Drosophila

Parkinson&#8217;s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, primarily in the substantia nigra. The disease causes motor deficiencies, which present as rigidity, tremors and dementia in humans. Rotenone is an insecticide that causes oxidative damage by inhibiting the function of the electron transport chain in mitochondria. It is also used to model Parkinson&#8217;s disease in the Drosophila. Flies have an inherent negative geotactic response, which compels them to climb upwards upon being startled. It has been established that rotenone causes early mortality and locomotion defects that disrupt the flies&#8217; ability to climb after they have been tapped downwards. However, the effect of rotenone on spontaneous movement is not well documented. This study outlines two sensitive, reproducible, and high throughput assays to characterize rotenone-induced deficiencies in short-term startle-induced locomotion and long-term spontaneous locomotion in Drosophila. These assays can be conveniently adapted to characterize other Drosophila models of locomotion defects and efficacy of therapeutic agents.

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson's Disease Model of Drosophila

Parkinson&#8217;s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, causing locomotion defects. Rotenone models Parkinson&#8217;s disease in Drosophila. This paper outlines two assays that characterize both spontaneous and startle-induced locomotion deficiencies caused by rotenone.

方法来描述自发和惊吓引起的步态中的鱼藤酮诱导的帕金森病模型<em&gt;果蝇</em

Parkinson&#8217;s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, ...

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神经科学

Measuring and Altering Mating Drive in Male Drosophila melanogaster

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, reductionist, and scalable system for in-depth investigation of motivation using the mating drive of male Drosophila&#160;melanogaster (Drosophila), the methods for which we detail here. The behavioral paradigm centers on the finding that male mating drive decreases alongside fertility over the course of repeated copulations and recovers over ~3 d. In this system, the powerful neurogenetic tools available in the fly converge with the genetic accessibility and putative wiring diagram available for sexual behavior. This convergence allows rapid isolation and interrogation of small neuronal populations with specific motivational functions. Here we detail the design and execution of the satiety assay that is used to measure and alter courtship motivation in the male fly. Using this assay, we also demonstrate that low male mating drive can be overcome by stimulating dopaminergic neurons. The satiety assay is simple, affordable, and robust to influences of genetic background. We expect the satiety assay to generate many new insights into the neurobiology of motivational states.

Measuring and Altering Mating Drive in Male Drosophila melanogaster

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

测量并改变配套驱动马累<em&gt;果蝇</em

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, ...

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

An increasing number of super-resolution microscopy techniques are helping to uncover the mechanisms that govern the nanoscale cellular world. Single-molecule imaging is gaining momentum as it provides exceptional access to the visualization of individual molecules in living cells. Here, we describe a technique that we developed to perform single-particle tracking photo-activated localization microscopy (sptPALM) in Drosophila larvae. Synaptic communication relies on key presynaptic proteins that act by docking, priming, and promoting the fusion of neurotransmitter-containing vesicles with the plasma membrane. A range of protein-protein and protein-lipid interactions tightly regulates these processes and the presynaptic proteins therefore exhibit changes in mobility associated with each of these key events. Investigating how mobility of these proteins correlates with their physiological function in an intact live animal is essential to understanding their precise mechanism of action. Extracting protein mobility with high resolution in vivo requires overcoming limitations such as optical transparency, accessibility, and penetration depth. We describe how photoconvertible fluorescent proteins tagged to the presynaptic protein Syntaxin-1A can be visualized via slight oblique illumination and tracked at the motor nerve terminal or along the motor neuron axon of the third instar Drosophila larva.

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

Here we illustrate how single molecule photo-activated localization microscopy can be carried out on the motor nerve terminal of a live Drosophila melanogaster third instar larva.

在体内果蝇前运动神经末端的单分子跟踪

An increasing number of super-resolution microscopy techniques are helping to uncover the mechanisms that govern the nanoscale cellular world. ...

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Dendrites are the branched projections of a neuron, and dendritic morphology reflects synaptic organization during the development of the nervous system. Drosophila larval neuronal dendritic arborization (da) is an ideal model for studying morphogenesis of neural dendrites and gene function in the development of nervous system. There are four classes of da neurons. Class IV is the most complex with a branching pattern that covers almost the entire area of the larval body wall. We have previously characterized the effect of silencing the Drosophila ortholog of SOX5 on class IV neuronal dendritic arborization complexity (NDAC) using four parameters: the length of dendrites, the surface area of dendrite coverage, the total number of branches, and the branching structure. This protocol presents the workflow of NDAC quantitative analysis, consisting of larval dissection, confocal microscopy, and image analysis procedures using ImageJ software. Further insight into da neuronal development and its underlying mechanisms will improve the understanding of neuronal function and provide clues about the fundamental causes of neurological and neurodevelopmental disorders.

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

This protocol focuses on quantitative analysis of neuronal dendritic arborization complexity (NDAC) in Drosophila, which can be used for studies of dendritic morphogenesis.

嗜德维人神经元树突状树突状复杂度的定量分析

Dendrites are the branched projections of a neuron, and dendritic morphology reflects synaptic organization during the development of the nervous system. ...

Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo

Use of the in vivo alpha-synuclein preformed fibril (&#945;-syn PFF) model of synucleinopathy is gaining popularity among researchers aiming to model Parkinson&#39;s disease synucleinopathy and nigrostriatal degeneration. The standardization of &#945;-syn PFF generation and in vivo application is critical in order to ensure consistent, robust &#945;-syn pathology. Here, we present a detailed protocol for the generation of fibrils from monomeric &#945;-syn, post-fibrilization quality control steps, and suggested parameters for successful neurosurgical injection of &#945;-syn PFFs into rats or mice. Starting with monomeric &#945;-syn, fibrilization occurs over a 7-day incubation period while shaking at optimal buffer conditions, concentration, and temperature. Post-fibrilization quality control is assessed by the presence of pelletable fibrils via sedimentation assay, the formation of amyloid conformation in the fibrils with a thioflavin T assay, and electron microscopic visualization of the fibrils. Whereas successful validation using these assays is necessary for success, they are not sufficient to guarantee PFFs will seed &#945;-syn inclusions in neurons, as such aggregation activity of each PFF batch should be tested in cell culture or in pilot animal cohorts. Prior to use, PFFs must be sonicated under precisely standardized conditions, followed by examination using electron microscopy or dynamic light scattering to confirm fibril lengths are within optimal size range, with an average length of 50 nm. PFFs can then be added to cell culture media or used in animals. Pathology detectable by immunostaining for phosphorylated &#945;-syn (psyn; serine 129) is apparent days or weeks later in cell culture and rodent models, respectively.&#160;

The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.

从单体和体内使用的Α-syr核 in 预成型纤维的产生

Use of the in vivo alpha-synuclein preformed fibril (&#945;-syn PFF) model of synucleinopathy is gaining popularity among researchers aiming to model ...

Applying the RatWalker System for Gait Analysis in a Genetic Rat Model of Parkinson's Disease

Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta. Gait abnormalities, including decreased arm swing, slower walking speed, and shorter steps are common in PD patients and appear early in the course of disease. Thus, the quantification of motor patterns in animal models of PD will be important for phenotypic characterization during disease course and upon therapeutic treatment. Most cases of PD are idiopathic; however, the identification of hereditary forms of PD uncovered gene mutations and variants, such as loss-of-function mutations in Pink1 and Parkin, two proteins involved in mitochondrial quality control that could be harnessed to create animal models. While mice are resistant to neurodegeneration upon loss of Pink1 and Parkin (single and combined deletion), in rats, Pink1 but not Parkin deficiency leads to nigral DA neuron loss and motor impairment. Here, we report the utility of FTIR imaging to uncover gait changes in freely walking young (2 months of age) male rats with combined loss of Pink1 and Parkin prior to the development of gross visually apparent motor abnormality as these rats age (observed at 4-6 months), characterized by hindlimb dragging as previously reported in Pink1 knockout (KO) rats.

Here we describe the RatWalker system, built by redesigning the MouseWalker apparatus to accommodate for the increased size and weight of rats. This system uses frustrated total internal reflection (FTIR), high-speed video capture, and open-access analysis software to track and quantify gait parameters.

将RatWalker系统应用于帕金森病遗传大鼠模型中的步态分析

Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by the loss of dopaminergic (DA) neurons in the substantia nigra pars ...

Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis

Estimation of the number of dopaminergic neurons in the substantia nigra is a key method in pre-clinical Parkinson's disease research. Currently, unbiased stereological counting is the standard for quantification of these cells, but it remains a laborious and time-consuming process, which may not be feasible for all projects. Here, we describe the use of an image analysis platform, which can accurately estimate the quantity of labeled cells in a pre-defined region of interest. We describe a step-by-step protocol for this method of analysis in rat brain and demonstrate it can identify a significant reduction in tyrosine hydroxylase positive neurons due to expression of mutant &#945;-synuclein in the substantia nigra. We validated this methodology by comparing with results obtained by unbiased stereology. Taken together, this method provides a time-efficient and accurate process for detecting changes in dopaminergic neuron number, and thus is suitable for efficient determination of the effect of interventions on cell survival.

Here we present an automated method for semi-quantitative determination of dopaminergic neuron number in the rat substantia nigra pars compacta.

使用自动图像分析对啮齿动物模型的亚斯坦蒂娅尼格拉多巴明神经元密度进行半定量测定

Estimation of the number of dopaminergic neurons in the substantia nigra is a key method in pre-clinical Parkinson's disease research. Currently, unbiased ...

The 6-hydroxydopamine Rat Model of Parkinson's Disease

Motor symptoms of Parkinson's disease (PD)-bradykinesia, akinesia, and tremor at rest-are consequences of the neurodegeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc) and dopaminergic striatal deficit. Animal models have been widely used to simulate human pathology in the laboratory. Rodents are the most used animal models for PD due to their ease of handling and maintenance. Moreover, the anatomy and molecular, cellular, and pharmacological mechanisms of PD are similar in rodents and humans. The infusion of the neurotoxin, 6-hydroxydopamine (6-OHDA), into a medial forebrain bundle (MFB) of rats reproduces the severe destruction of dopaminergic neurons and simulates PD symptoms. This protocol demonstrates how to perform the unilateral microinjection of 6-OHDA in the MFB in a rat model of PD and shows the motor deficits induced by 6-OHDA and predicted dopaminergic lesions through the stepping test. The 6-OHDA causes significant impairment in the number of steps performed with the contralateral forelimb.

The 6-hydroxydopamine (6-OHDA) model has been used for decades to advance the understanding of Parkinson's Disease. In this protocol, we demonstrate how to perform unilateral nigrostriatal lesions in the rat by injecting 6-OHDA in the medial forebrain bundle, assess motor deficits, and predict lesions using the stepping test.

帕金森病的6-羟基多巴胺大鼠模型

Motor symptoms of Parkinson's disease (PD)-bradykinesia, akinesia, and tremor at rest-are consequences of the neurodegeneration of dopaminergic neurons in ...

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

Amyotrophic lateral sclerosis is a neurodegenerative disorder causing progressive muscle weakness and death within 2-5 years following diagnosis. Clinical manifestations include weight loss, dyslipidemia, and hypermetabolism; however, it remains unclear how these relate to motor neuron degeneration. Using a Drosophila model of TDP-43 proteinopathy that recapitulates several features of ALS including cytoplasmic inclusions, locomotor dysfunction, and reduced lifespan, we recently identified broad ranging metabolic deficits. Among these, glycolysis was found to be upregulated and genetic interaction experiments provided evidence for a compensatory neuroprotective mechanism. Indeed, despite upregulation of phosphofructokinase, the rate limiting enzyme in glycolysis, an increase in glycolysis using dietary and genetic manipulations was shown to mitigate locomotor dysfunction and increased lifespan in fly models of TDP-43 proteinopathy. To further investigate the effect on TDP-43 proteinopathy on glycolytic flux in motor neurons, a previously reported genetically encoded, FRET-based sensor, FLII12Pglu-700&#181;&#948;6, was used. This sensor is comprised of a bacterial glucose-sensing domain and cyan and yellow fluorescent proteins as the FRET pair. Upon glucose binding, the sensor undergoes a conformational change allowing FRET to occur. Using FLII12Pglu-700&#181;&#948;6, glucose uptake was found to be significantly increased in motor neurons expressing TDP-43G298S, an ALS causing variant. Here, we show how to measure glucose uptake, ex vivo, in larval ventral nerve cord preparations expressing the glucose sensor FLII12Pglu-700&#181;&#948;6 in the context of TDP-43 proteinopathy. This approach can be used to measure glucose uptake and assess glycolytic flux in different cell types or in the context of various mutations causing ALS and related neurodegenerative disorders.

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

Glucose uptake is increased in Drosophila motor neurons affected by TAR DNA binding protein (TDP-43) proteinopathy, as indicated by a FRET-based, genetically encoded glucose sensor.

测量TDP-43蛋白病果 蝇 模型中的葡萄糖摄取

Amyotrophic lateral sclerosis is a neurodegenerative disorder causing progressive muscle weakness and death within 2-5 years following diagnosis. Clinical ...

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson's Disease

Levodopa (L-DOPA) remains the gold-standard therapy used to treat Parkinson&#39;s disease (PD) motor symptoms. However, unwanted involuntary movements known as L-DOPA-induced dyskinesias (LIDs) develop with prolonged use of this dopamine precursor.&#160;It is estimated that the incidence of LIDs escalates to approximately 90% of individuals with PD within 10&#8211;15 years of treatment. Understanding the mechanisms of this malady and developing both novel and effective anti-dyskinesia treatments requires consistent and accurate modeling for pre-clinical testing of therapeutic interventions. A detailed method for reliable induction and comprehensive rating of LIDs following 6-OHDA-induced nigral lesioning in a rat model of PD is presented here. Dependable LID assessment in rats provides a powerful tool that can be readily utilized across laboratories to test emerging therapies focused on reducing or eliminating this common treatment-induced burden for individuals with PD.

This article describes methods to induce and evaluate levodopa-induced dyskinesias in a rat model of Parkinson&#39;s disease. The protocol offers detailed information regarding the intensity and frequency of a range of dyskinetic behaviors, both dystonic and hyperkinetic, providing a reliable tool to test treatments targeting this unmet medical need.

左旋多巴诱导的帕金森病大鼠模型中运动障碍的诱导与评估

Levodopa (L-DOPA) remains the gold-standard therapy used to treat Parkinson&#39;s disease (PD) motor symptoms. However, unwanted involuntary movements ...

在帕金森病的果蝇模型中测量便秘

副本

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此视频中的章节

方法来描述自发和惊吓引起的步态中的鱼藤酮诱导的帕金森病模型

测量并改变配套驱动马累

在体内果蝇前运动神经末端的单分子跟踪

嗜德维人神经元树突状树突状复杂度的定量分析

从单体和体内使用的Α-syr核 in 预成型纤维的产生

将RatWalker系统应用于帕金森病遗传大鼠模型中的步态分析

使用自动图像分析对啮齿动物模型的亚斯坦蒂娅尼格拉多巴明神经元密度进行半定量测定

帕金森病的6-羟基多巴胺大鼠模型

测量TDP-43蛋白病果蝇模型中的葡萄糖摄取

左旋多巴诱导的帕金森病大鼠模型中运动障碍的诱导与评估

在帕金森病的 果蝇 模型中测量便秘

副本

摘要

探索更多视频

此视频中的章节

方法来描述自发和惊吓引起的步态中的鱼藤酮诱导的帕金森病模型

测量并改变配套驱动马累

在体内果蝇前运动神经末端的单分子跟踪

嗜德维人神经元树突状树突状复杂度的定量分析

从单体和体内使用的Α-syr核 in 预成型纤维的产生

将RatWalker系统应用于帕金森病遗传大鼠模型中的步态分析

使用自动图像分析对啮齿动物模型的亚斯坦蒂娅尼格拉多巴明神经元密度进行半定量测定

帕金森病的6-羟基多巴胺大鼠模型

测量TDP-43蛋白病果 蝇 模型中的葡萄糖摄取

左旋多巴诱导的帕金森病大鼠模型中运动障碍的诱导与评估

在帕金森病的果蝇模型中测量便秘

测量TDP-43蛋白病果蝇模型中的葡萄糖摄取