<meta charset="utf8"></head><body>建立三叉神经性疼痛的小鼠模型

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	<li>Costigan, M., Scholz, J., Woolf, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropathic+pain:+a+maladaptive+response+of+the+nervous+system+to+damage.">Neuropathic pain: a maladaptive response of the nervous system to damage.</a> Annu Rev Neurosci. 32, 1-32 (2009).</li><li>Torrance, N., Smith, B. H., Bennett, M. I., Lee, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+epidemiology+of+chronic+pain+of+predominantly+neuropathic+origin.+Results+from+a+general+population+survey.">The epidemiology of chronic pain of predominantly neuropathic origin. Results from a general population survey.</a> J Pain. 7, 281-289 (2006).</li><li>Jensen, T. S., Gottrup, H., Sindrup, S. H., Bach, F. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+clinical+picture+of+neuropathic+pain.">The clinical picture of neuropathic pain.</a> Eur J Pharmacol. 429 (1-3), 1-11 (2001).</li><li>Bennett, G. J., Xie, Y. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+peripheral+mononeuropathy+in+rat+that+produces+disorders+of+pain+sensation+like+those+seen+in+man.">A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man.</a> Pain. 33 (1), 87-107 (1988).</li><li>Backonja, M. M., Stacey, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuropathic+pain+symptoms+relative+to+overall+pain+rating.">Neuropathic pain symptoms relative to overall pain rating.</a> J Pain. 5, 491-497 (2004).</li><li>Basbaum, A. I., Campbell, J. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurrement+and+New+Technologies.">Measurrement and New Technologies.</a> Emerging Strategies for the Treatment of Neuropathic Pain. , (2006).</li><li>Mogil, J. S., Crager, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What+should+we+be+measuring+in+behavioral+studies+of+chronic+pain+in+animals.">What should we be measuring in behavioral studies of chronic pain in animals.</a> Pain. 112, 12-15 (2004).</li><li>Vierck, C. J., Campbell, J. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+Studies+of+Pain:+Lessons+for+Drug+Development.">Animal Studies of Pain: Lessons for Drug Development.</a> Emerging Strategies for the Treatment of Neuropathic Pain. , (2006).</li><li>Deseure, K., Adriaensen, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonevoked+facial+pain+in+rats+following+infraorbital+nerve+injury+a+parametric+analysis.">Nonevoked facial pain in rats following infraorbital nerve injury a parametric analysis.</a> Physiol Behav. 81 (4), 595-604 (2004).</li><li>Deseure, K., Hans, G. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+constriction+injury+of+the+rat's+infraorbital+nerve+(IoN-CCI)+to+study+trigeminal+neuropathic+pain.">Chronic constriction injury of the rat's infraorbital nerve (IoN-CCI) to study trigeminal neuropathic pain.</a> J Vis Exp. (103), e53167 (2015).</li><li>Mogil, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+pain:+progress+and+challenges.">Animal models of pain: progress and challenges.</a> Nat Rev Neurosci. 10 (4), 283-294 (2009).</li><li>Wilson, S. G., Mogil, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+pain+in+the+(knockout)+mouse:+big+challenges+in+a+small+mammal.">Measuring pain in the (knockout) mouse: big challenges in a small mammal.</a> Behav Brain Res. 125 (1-2), 65-73 (2001).</li><li>Ding, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+rodent+model+of+trigeminal+neuropathic+pain+by+unilateral+chronic+constriction+injury+of+distal+infraorbital+nerve.">An improved rodent model of trigeminal neuropathic pain by unilateral chronic constriction injury of distal infraorbital nerve.</a> J Pain. 18 (8), 899-907 (2017).</li><li>Hardt, S., Fischer, C., Vogel, A., Wilken-Schmitz, A., Tegeder, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distal+infraorbital+nerve+injury:+a+model+for+persistent+facial+pain+in+mice.">Distal infraorbital nerve injury: a model for persistent facial pain in mice.</a> Pain. 160 (6), 1431-1447 (2019).</li><li>Krzyzanowska, A., Avenda&#241;o, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+testing+in+rodent+models+of+orofacial+neuropathic+and+inflammatory+pain.">Behavioral testing in rodent models of orofacial neuropathic and inflammatory pain.</a> Brain Behav. 2 (5), 678-697 (2012).</li><li>Martin, Y. B., Malmierca, E., Avenda&#241;o, C., Nu&#241;ez, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuronal+disinhibition+in+the+trigeminal+nucleus+caudalis+in+a+model+of+chronic+neuropathic+pain.">Neuronal disinhibition in the trigeminal nucleus caudalis in a model of chronic neuropathic pain.</a> Eur J Neurosci. 32 (3), 399-408 (2010).</li><li>Deseure, K., Hans, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+study+of+non+evoked+orofacial+pain+following+different+types+of+infraorbital+nerve+injury+in+rats.">Behavioral study of non evoked orofacial pain following different types of infraorbital nerve injury in rats.</a> Physiol Behav. 138, 292-296 (2015).</li><li>Vuralli, D., Wattiez, A. S., Russo, A. F., Bolay, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+and+cognitive+animal+models+in+headache+research.">Behavioral and cognitive animal models in headache research.</a> J Headache Pain. 20 (1), 11 (2019).</li><li>Krzyzanowska, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessing+nociceptive+sensitivity+in+mouse+models+of+inflammatory+and+neuropathic+trigeminal+pain.">Assessing nociceptive sensitivity in mouse models of inflammatory and neuropathic trigeminal pain.</a> J Neurosci Methods. 201 (1), 46-54 (2011).</li></ol>

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			<td>Chromic catgut (6-0)</td>
			<td>Dynek&#160;</td>
			<td>CG602D</td>
			<td>ligatures</td>
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		<tr>
			<td>Cotton applicator</td>
			<td>Pharmacy</td>
			<td></td>
			<td></td>
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			<td>Digital video camera</td>
			<td>Sony</td>
			<td>HDR-CX330E</td>
			<td></td>
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		<tr>
			<td>Dumont #5 forceps</td>
			<td>Fine Science Tools</td>
			<td>11251-10</td>
			<td></td>
		</tr>
		<tr>
			<td>Dumont forceps - Micro-blunted tips (#5/45)</td>
			<td>Fine Science Tools</td>
			<td>11253-25</td>
			<td></td>
		</tr>
		<tr>
			<td>Duratears</td>
			<td>Alcon</td>
			<td>0037-820</td>
			<td>ophthalmic ointment</td>
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			<td>Hooked ligation aid</td>
			<td>Fine Science Tools</td>
			<td>18062-12</td>
			<td></td>
		</tr>
		<tr>
			<td>Ketalar</td>
			<td>Pfizer</td>
			<td></td>
			<td>ketamine (50 mg/mL)</td>
		</tr>
		<tr>
			<td>Operation microscope</td>
			<td>Kaps</td>
			<td>SOM 62</td>
			<td></td>
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		<tr>
			<td>Precision cotton swab</td>
			<td>Qosina</td>
			<td>10225</td>
			<td></td>
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		<tr>
			<td>Precision trimmer</td>
			<td>Philips</td>
			<td>HP6392/00</td>
			<td></td>
		</tr>
		<tr>
			<td>Rompun</td>
			<td>Bayer</td>
			<td></td>
			<td>xylazine (2%)</td>
		</tr>
		<tr>
			<td>Scissors - blunt tips</td>
			<td>Fine Science Tools</td>
			<td>14574-09</td>
			<td></td>
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		<tr>
			<td>Semmes-Weinstein Von Frey Aesthesiometer kit</td>
			<td>Stoelting</td>
			<td>58011</td>
			<td></td>
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		<tr>
			<td>Vicryl Rapide</td>
			<td>Ethicon</td>
			<td>MPVR489H</td>
			<td>sutures</td>
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</table>

chronic constriction injury of the distal infraorbital nerve (dion-cci) in mice to study trigeminal neuropathic pain

Neuropathic pain arises from damage to the somatosensory nervous system, leading to abnormal transmission of sensory signals to the brain. Somatosensory nerve damage does not always lead to neuropathic pain, but the prevalence increases with the severity of clinical neuropathy1,2. Neuropathic pain patients experience specific symptoms such as spontaneous sensations (burning, pins and needles, electric sensations) and abnormally intense or prolonged pain to innocuous or noxious stimulation that tend to become chronic and resistant to treatment with conventional pain medication3. Significant progress in the field of neuropathic pain research stems from the discovery that loosely constricting ligatures around the sciatic nerve in rats leads to behaviors resembling human neuropathic pain conditions4. The animals display reduced thresholds to heat, cold, and mechanical stimulation, and exhibit nocifensive behaviors. Despite the inherent biological differences in pain processing between humans and rodents, animal models are a valuable tool for studying the underlying mechanisms in the development of neuropathic pain and testing newly proposed treatment strategies.
Sensory reflex-based pain testing paradigms have been extensively used in neuropathic pain models, but measuring ongoing pain or other frequently accompanied disturbances (sleeping disorder, depression, anxiety) has not received sufficient attention considering that these are common clinical symptoms affecting quality of life5,6,7,8. Face grooming behavior in rats has been documented as a measure of spontaneous neuropathic pain following chronic constriction injury (CCI) of the infraorbital nerve (IoN)9,10. In addition, rats also develop hyperresponsiveness to mild tactile stimulation of the IoN territory, which is indicative of mechanical allodynia.
Compared to mice, because of their larger size, rats are better suited for surgical injuries. However, mice offer cost and space efficiency and require smaller drug quantities. Also, the advent of transgenic technology has further boosted the use of mice11,12. Therefore, the overall goal of this procedure is to perform a surgical infraorbital nerve injury in mice, similar to that in rats, that induces changes in spontaneous and evoked behavior for the study of trigeminal neuropathic pain.

<meta charset="utf8"></head><body>作者聚焦：利用小鼠眶下神经结扎术研究三叉神经性疼痛和治疗策略

小鼠远端眶下神经慢性缩窄损伤 （DIoN-CCI） 研究三叉神经性疼痛

The overall goal of this procedure is to perform an infraorbital nerve injury in mice to study the development of trigeminal neuropathic pain and to test new treatment strategies. Isolated phase grooming behavior after infraorbital nerve ligation is one of the few animal models to study spontaneous neuropathic pain. But its use has long been hampered by the complexity of performing an infraorbital nerve ligation.Compared to sensory reflex based pain testing, such as from fray or withdrawal responses from heat or cold stimulation, measuring spontaneous or ongoing pain has not received sufficient attention, considering that this is an important clinical symptom affecting quality of life. The distal approach to the infraorbital nerve has the advantage of being a surgically less demanding procedure than the intraorbital approach. It is minimally invasive, and can be performed without using a stereotaxic frame.

Watch this Scientific Journal Video about Utilizing Infraorbital Nerve Ligation in Mice for Investigating Trigeminal Neuropathic Pain and Treatment Strategies at JoVE.com

Animal models remain necessary tools to study neuropathic pain. This manuscript describes the distal infraorbital nerve chronic constriction injury ...

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Neuroscience

Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain

682 次观看.  University of Antwerp. 该程序的总体目标是对小鼠进行眶下神经损伤，以研究三叉神经性疼痛的发展并测试新的治疗策略。眶下神经结扎后的孤立期梳理行为是研究自发性神经性疼痛的少数动物模型之一。但长期以来，由于进行眶下神经结扎术的复杂性，它的使用一直受到阻碍。与基于感觉反射的疼痛测试相比，例如来自热或冷刺激的磨损或戒断反应，考虑到这是影...