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各种研究表明，减少交付脑内注射，组织创伤和侮辱血脑屏障套管的大小减少（佩里等人，1993年），炎症减少，免疫反应减弱（Finsen等人减少，1991），反应性胶质化（Nikkhah等，1994）。我们在座的一种neuroactive代理大脑内的慢性交付通过一个直径6倍，比常规使用的交付插管，减少microcannula方法。我们已经证明，该系统可靠地提供了一个有代表性的解决方案随着时间的推移和局部创伤和反应性胶质化水平显着降低...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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<td>Mini-osmotic pumps (MOPs)</td>
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<td>World Precision Instruments</td>
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<td>Microforceps</td>
<td>Surgery</td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

慢性滴注neuroactive代理使用的大脑区域内的实验协议，通常利用一个微型渗透泵系统。代理通常是通过一个直径为0.30毫米或更大的不锈钢套管交付。利用这一口径套管的系统，可以并处创伤导致建筑损坏的利益方面，从而影响结构的完整性和正常运作。随着神经科学的查询变得更加复杂，调查的大脑结构和电路需要提高精度和更高的分辨率的水平。我们已经开发出一种利用与尖端直径为50微米的高硼硅microcannula了大脑内的慢性输液系统的准备和植入的方法。这种技术减少了对当地环境的破坏和减少输液部位的反应性胶质化。 microinfusion系统的配置也能够以符合动物的头骨表面，解除对大脑神经底座的需要，从而促进头皮切口封闭和减少感染的风险。我们表现​​出持续提供可靠的一个有代表性的分子量使用体外模型，并在大鼠体内研究的染料。

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

随着神经科学的查询变得更加复杂，调查的大脑结构和电路需要提高精度和更高的分辨率的水平。我们已经开发出一种利用与尖端直径为50微米的高硼硅microcannula了大脑内的慢性输液系统的准备和植入的方法。

为持续给药Neuroactive代理Microinfusion系统的建设和植入。

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

construction-implantation-microinfusion-system-for-sustained-delivery

Research

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Biology

15.4K 次观看.  Harvard Medical School. 随着神经科学的查询变得更加复杂，调查的大脑结构和电路需要提高精度和更高的分辨率的水平。我们已经开发出一种利用与尖端直径为50微米的高硼硅microcannula了大脑内的慢性输液系统的准备和植入的方法。