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Diverses études ont démontré qu&#39;en diminuant la taille de la canule de livraison pour les perfusions intracérébrales, d&#39;un traumatisme tissulaire et insulte à la barrière hémato-encéphalique sont réduits (Perry et al., 1993), l&#39;inflammation est diminuée et la réponse immunitaire atténuée (Finsen et al gliose., 1991), et réactive est diminuée (Nikkhah et al., 1994). Nous présentons ici une méthode pour la livraison d&#39;agents neuro chroniques dans le cerveau via un microcanule avec un diamètre qui est réduit...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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<td>Mini-osmotic pumps (MOPs)</td>
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<td>Microforceps</td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

Les protocoles expérimentaux utilisés pour perfusion chronique d&#39;agents neuro au sein des régions du cerveau utilisent souvent un système de pompe mini-osmotique. Les agents sont généralement livrés par une canule en acier inoxydable d&#39;un diamètre de 0,30 mm ou plus. Systèmes utilisant une canule de ce calibre peut imposer un traumatisme à la zone d&#39;intérêt résultant des dommages architecturaux, compromettant ainsi l&#39;intégrité structurelle et le fonctionnement normal. Comme neurosciences enquête devient plus sophistiquée, l&#39;enquête sur les structures du cerveau et des circuits exige des niveaux améliorés de précision et une résolution plus élevée. Nous avons développé une méthode pour la préparation et l&#39;implantation d&#39;un système de perfusion chronique dans le cerveau en utilisant un microcanule borosilicate avec un diamètre de pointe de 50 microns. Cette technique réduit les dommages à l&#39;environnement local et diminue gliose réactive sur le site de perfusion. La configuration du système de microperfusion est aussi capable de s&#39;adapter à la surface du crâne de l&#39;animal, écartant la nécessité pour les grands socles crânienne, facilitant ainsi la fermeture de l&#39;incision du cuir chevelu et de réduire le risque d&#39;infection. Nous démontrons fiabilité de livraison soutenue d&#39;un colorant ayant un poids moléculaire en utilisant représentant un modèle in vitro et in vivo chez le rat.

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

Comme neurosciences enquête devient plus sophistiquée, l&#39;enquête sur les structures du cerveau et des circuits exige des niveaux améliorés de précision et une résolution plus élevée. Nous avons développé une méthode pour la préparation et l&#39;implantation d&#39;un système de perfusion chronique dans le cerveau en utilisant un microcanule borosilicate avec un diamètre de pointe de 50 microns.

Construction et implantation d&#39;un système de livraison microperfusion soutenue des agents neuroactifs.

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

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15.4K vues.  Harvard Medical School. Comme neurosciences enquête devient plus sophistiquée, l'enquête sur les structures du cerveau et des circuits exige des niveaux améliorés de précision et une résolution plus élevée. Nous avons développé une méthode pour la préparation et l'implantation d'un système de perfusion chronique dans le cerveau en utilisant un microcanule borosilicate avec un diamètre de pointe de 50 microns.