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Vários estudos têm demonstrado que, diminuindo o tamanho da cânula de entrega para infusões intracerebrais, trauma tecidual e insulto à barreira hemato-encefálica são reduzidos (Perry et al., 1993), a inflamação é diminuído eo atenuada imuno-resposta (Finsen et al gliose., 1991), e reativa é diminuída (Nikkhah et al., 1994). Apresentamos aqui um método para a entrega crônica de agentes neuroactive dentro do cérebro através de uma microcânula, com um diâmetro que é reduzida de 6 vezes comparado ao de cânulas de entrega c...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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<td>Mini-osmotic pumps (MOPs)</td>
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<td>model #1002</td>
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<td>Electrode puller</td>
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<td>Stoelting Co.</td>
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<td>World Precision Instruments</td>
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<td>Microforceps</td>
<td>Surgery</td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

Protocolos experimentais usados ​​para infusão crônica de agentes neuroactive dentro das regiões do cérebro, muitas vezes utilizam um sistema de bomba de mini-osmótica. Agentes são comumente entregues através de uma cânula de aço inoxidável com um diâmetro de 0,30 mm ou maior. Sistemas utilizando uma cânula deste calibre pode impor trauma para a área de interesse, resultando em danos arquitetônicos, comprometendo a integridade estrutural e funcionamento normal. Como neurociência inquérito se torna mais sofisticada investigação, de estruturas cerebrais e circuitos exige melhores níveis de precisão e de alta resolução. Nós desenvolvemos um método para a preparação e implantação de um sistema de infusão crônica no cérebro utilizando uma microcânula borosilicato com um diâmetro da ponta de 50 microns. Esta técnica reduz os danos ao meio ambiente local e diminui gliose reativa no local da infusão. A configuração do sistema microinfusion também é capaz de estar de acordo com a superfície do crânio do animal, impedindo a necessidade de grandes pedestais cranial, facilitando o fechamento da incisão no couro cabeludo e reduzindo o risco de infecção. Demonstramos entrega sustentado confiável de um corante tendo um peso representativo molecular utilizando um modelo in vitro e in vivo em ratos.

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

Como neurociência inquérito se torna mais sofisticada investigação, de estruturas cerebrais e circuitos exige melhores níveis de precisão e de alta resolução. Nós desenvolvemos um método para a preparação e implantação de um sistema de infusão crônica no cérebro utilizando uma microcânula borosilicato com um diâmetro da ponta de 50 microns.

Construção e Implantação de um Sistema de Microinfusion para entrega Sustentada de Agentes neuroativos.

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

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15.4K visualizações.  Harvard Medical School. Como neurociência inquérito se torna mais sofisticada investigação, de estruturas cerebrais e circuitos exige melhores níveis de precisão e de alta resolução. Nós desenvolvemos um método para a preparação e implantação de um sistema de infusão crônica no cérebro utilizando uma microcânula borosilicato com um diâmetro da ponta de 50 microns.