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Verschiedene Studien belegen, dass durch die Verringerung der Größe der Lieferung Kanüle für intrazerebrale Infusionen, Gewebetrauma und Beleidigung für die Blut-Hirn-Schranke reduziert werden (Perry et al., 1993), ist eine Entzündung verringert und die Immunantwort abgeschwächt (Finsen et al ., 1991) und reaktive Gliose ist vermindert (Nikkhah et al., 1994). Wir stellen hier ein Verfahren für die chronische Lieferung von neuroaktiven Agenten innerhalb des Gehirns über eine Mikrokanüle mit einem Durchmesser, 6-fach, um die von her...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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<td>Mini-osmotic pumps (MOPs)</td>
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<td>Microforceps</td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

Experimental-Protokolle für die chronische Infusion von neuroaktiven Agenten in Regionen des Gehirns eingesetzt nutzen oft eine Mini-osmotischen Pumpe-System. Agents werden in der Regel über eine Kanüle aus rostfreiem Stahl mit einem Durchmesser von 0,30 mm oder mehr geliefert. Systeme unter Verwendung einer Kanüle von diesem Kaliber kann Trauma im Bereich der Zinsen, die sich in Architektur Schaden zu verhängen, damit Kompromisse strukturelle Integrität und das normale Funktionieren. Als neurowissenschaftliche Untersuchung wird immer anspruchsvoller, die Untersuchung von Hirnstrukturen und-Schaltung erfordert eine verbesserte Genauigkeit und eine höhere Auflösung. Wir haben ein Verfahren zur Herstellung und Implantation einer chronischen Infusion System im Gehirn unter Verwendung einer Borosilikat Mikrokanüle mit einer Spitze Durchmesser von 50 Mikrometer entwickelt. Diese Technik reduziert den Schaden für die lokale Umwelt und verringert reaktive Gliose am Ort der Infusion. Die Konfiguration der Mikroinjektion ist auch in der Lage, auf der Oberfläche des Tieres Schädel entsprechen, unter Ausschluss der Bedarf an großen kranialen Sockeln und erleichtert so Schließung der Kopfhaut Schnitt und reduzieren das Risiko einer Infektion. Wir zeigen zuverlässig anhaltende Abgabe eines Farbstoffes mit einem Vertreter Molekulargewicht mit einem in vitro-Modell und in vivo Studien an Ratten.

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

Als neurowissenschaftliche Untersuchung wird immer anspruchsvoller, die Untersuchung von Hirnstrukturen und-Schaltung erfordert eine verbesserte Genauigkeit und eine höhere Auflösung. Wir haben ein Verfahren zur Herstellung und Implantation einer chronischen Infusion System im Gehirn unter Verwendung einer Borosilikat Mikrokanüle mit einer Spitze Durchmesser von 50 Mikrometer entwickelt.

Bau-und Implantation eines Mikroinfusion System für Nachhaltige Lieferung von Neuroactive Agents.

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

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15.4K Aufrufe.  Harvard Medical School. Als neurowissenschaftliche Untersuchung wird immer anspruchsvoller, die Untersuchung von Hirnstrukturen und-Schaltung erfordert eine verbesserte Genauigkeit und eine höhere Auflösung. Wir haben ein Verfahren zur Herstellung und Implantation einer chronischen Infusion System im Gehirn unter Verwendung einer Borosilikat Mikrokanüle mit einer Spitze Durchmesser von 50 Mikrometer entwickelt.