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Shenzhen Bay laboratory

7 ARTICLES PUBLISHED IN JoVE

Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Yan Wu¹, Xiang Qian¹, Shengli Mi¹, Min Zhang¹, Shuqing Sun¹, Xiaohao Wang^1,2

¹Graduate School at Shenzhen, Tsinghua University, ²The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

Chemistry

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Xiaodong Shi^*1, Yinghuan Liu^*1, Rongtong Zhao¹, Zigang Li¹

¹Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School

We present a protocol for the construction of thioether/vinyl sulfide-tethered helical peptides using photo-induced thiol-ene/thiol-yne hydrothiolation.

JoVE Core

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Lingxiao Chaihu^1,2, Xiaopeng Yao¹, Xiang Xu^1,2, Zhongqin Zhu¹, David D. Y. Chen³, Guanbo Wang¹

¹School of Chemistry and Materials Science, Nanjing Normal University, ²Institute for Cell Analysis, Shenzhen Bay Laboratory, ³Department of Chemistry, University of British Columbia

Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.

Behavior

Individualized rTMS Treatment for Depression using an fMRI-Based Targeting Method

Xin Luo¹, Yiru Hu¹, Runhua Wang¹, Min Zhang¹, Xiaomei Zhong¹, Bin Zhang¹

¹Affiliated Brain Hospital of Guangzhou Medical University

The present protocol describes the application of repetitive transcranial magnetic stimulation (rTMS), where a subregion of the dorsolateral prefrontal cortex (DLPFC) with the strongest functional anticorrelation with the subgenual anterior cingulate cortex (sgACC) was located as the stimulation target under the assistance of a fMRI-based neuronavigation system.

Biology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Qiang Yan^*1, Yingying He^*2,3, Yang Yue^2,3, Luyao Jie¹, Tingmei Wen^2,3, Yumiao Zhao^2,3, Min Zhang², Tingting Zhang²

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, ²Research Institute of Applied Biology, Shanxi University, ³School of Life Science, Shanxi University

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Chunli Song^*1, Zhanfeng Hou^*1,2, Zijun Jiao^*1, Zhaodi Liu³, Chenshan Lian^1,2, Min Zhang¹, Wei Liang³, Feng Yin^1,2, Zigang Li^1,2

¹Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, ²Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, ³Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

Cancer Research

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Huayun Yan¹, Yingfang Ma¹, Xinyue Zhou², Yushuang He³, Yang Liu², Carlos Caulin⁴, Leiming Wang⁵, Heng Xu^1,6, Han Luo^2,6

¹State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, ²Division of Thyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-Related Molecular Network, Department of General Surgery, West China Hospital, Sichuan University, ³Department of Ultrasound, West China Hospital of Sichuan University, Sichuan University, ⁴Department of Otolaryngology-Head & Neck Surgery and University of Arizona Cancer Center, University of Arizona, ⁵Center for Translational Medicine, Shenzhen Bay Laboratory, ⁶Division of Laboratory Medicine/Research Centre of Clinical Laboratory Medicine, West China Hospital, Sichuan University

Here, we present a standard pipeline to obtain murine ATC tumors by spontaneous genetically engineered mouse models. Further, we present tumor dynamics and pathological information about the primary and metastasized lesions. This model will help researchers to understand tumorigenesis and facilitate drug discoveries.