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JoVE Journal

Medicine

This content is Open Access.

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging

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08:04 min

June 21st, 2011

June 21st, 2011

18,032 Views

0:05

Title

0:21

Subscription

1:38

Inoculation of Tumors into Avian Embryo CAM

3:10

Preparation of Nanoparticles

3:52

Intravenous Injection of Fluorescent Nanoparticles

5:00

Real Time Intravital Imaging and Quantification

6:47

Uptake of CPMV-PEG into a CAM Tumor

7:35

Conclusion

We present a novel approach to quantify nanoparticle localization in the vasculature of human xenografted tumors using dynamic, real-time intravital imaging in an avian embryo model.

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