<a href="https://www.jove.com/video/4189">LabVIEW-betriebenen Novel Nanoliter Osmometer für Ice Binding Protein Untersuchungen</a> Ido Braslavsky 1, 2, Ran Drori 1 1 Institut für Biochemie, Lebensmittelchemie und Ernährung, der Robert H. Smith Fakultät für Landwirtschaft, Ernährung und Umwelt, The Hebrew University of Jerusalem, 2 Department of Physics and Astronomy, Ohio University Eis Bindungsproteine ​​(IBPs), auch als Frostschutzmittel Proteine ​​bekannt, hemmen Eiswachstum und sind eine vielversprechende Additiv zur Verwendung bei der Kryokonservierung von Gewebe. Das wichtigste Instrument zur IBPs zu untersuchen ist die Nanoliter Osmometer. Wir haben ein Haus entworfen Kühlstufe auf einem optischen Mikroskop montiert und kontrolliert über eine custom-built LabVIEW Routine. Die Nanoliter Osmometer hier beschriebenen manipulierten die Temperatur der Probe in einem ultra-sensitive Art und Weise. <ahref = &quot;https://www.jove.com/video/50022&quot;&gt; Origami Inspiriert Selbstorganisation von Patterned und Reconfigurable Particles Shivendra Pandey 1, Evin Gultepe 1, David H. Gracias 1, 2 1 Department für Chemische und Biomolekulare Ingenieurwissenschaften, The Johns Hopkins University, 2 Department of Chemistry, The Johns Hopkins University Wir beschreiben experimentellen Details der Synthese von gemusterten und rekonfigurierbare Teilchen von zweidimensionalen (2D) Vorstufen. Diese Methodik kann verwendet werden, um Partikel in einer Vielzahl von Formen, einschließlich Polyeder und Greifvorrichtungen auf Längenskalen Bereich von Mikro-bis Zentimeterbereich erzeugen. <a href="https://www.jove.com/video/50225">Schnelle kolorimetrische Assays qualitativ unterscheiden RNA und DNA in Biomolekulare Proben</a> Jennifer Patterson, Cameron Mura Abteilungsübergreifendenent of Chemistry, University of Virginia Eine Reihe von kolorimetrischen Assays wird zum schnellen Unterscheidungsmerkmal Protein, RNA, DNA und Reduk-tion Zuckern in potentiell heterogenen biomolekularen Proben beschrieben. <a href="https://www.jove.com/video/4323">Herstellung und Verwendung von Samariumdiiodid (SmI 2) in der organischen Synthese: die mechanistische Rolle von HMPA und Ni (II)-Salzen in Samarium Barbier Reaction</a> Dhandapani V. Sadasivam, Kimberly A. Choquette, Robert A. Flowers II Department of Chemistry, Lehigh University Ein unkompliziertes Verfahren zur Herstellung Samariumdiiodid (SmI 2) in THF wird beschrieben. Die Rolle der beiden wichtigsten Zusatzstoffe nämlich Hexamethylphosphorsäuretriamid (HMPA) und Ni (acac) 2 in Sm-vermittelte Reaktionen in der Sm-Barbier-Reaktion nachgewiesen. <a href="https://www.jove.com/video/50021">Bringing</a>Das sichtbare Universum in Focus mit Robo-AO Christoph Baranec 1, 2, Reed Riddle 1, Nicholas M. Law 3, AN Ramaprakash 4, Shriharsh P. Tendulkar 2, Khanh Bui 1, Mahesh P. Burse 4, Pravin Chordia 4, Hillol K. Das 4, Jack TC Davis 1 , Richard G. Dékány 1, Mansi M. Kasliwal 5, Shrinivas R. Kulkarni 1, 2, Timothy D. Morton 2, Eran O. Ofek 6, Sujit Punnadi 4 1 Caltech Optical Observatorien, California Institute of Technology, 2 Department of Astronomy, California Institute of Technology, 3 Dunlap Institut für Astronomie und Astrophysik, Universität Toronto, 4 Inter-University Centre for Astronomy &amp; Astrophysics, 5 Beobachtungsstellen der Carnegie Institution for Science , 6 Benoziyo Center for Astrophysics, Weizmann<html> Institute of Science Licht aus astronomischer Objekte müssen über der Erde turbulente Atmosphäre zu reisen, bevor es von bodengebundenen Teleskopen abgebildet werden können. Um die direkte Bildgebung mit maximaler theoretischer Winkelauflösung zu ermöglichen, müssen fortgeschrittenen Techniken, wie sie von der Robo-AO-adaptive Optik eingesetzt werden. <a href="https://www.jove.com/video/50130">Bahndaten Analysen für Fußgänger Raum-Zeit-Activity Study</a> Feng Qi 1, Fei Du 2 1 School of Environmental and Life Sciences, Kean University, 2 Department of Geography, University of Wisconsin-Madison Eine Folge von Raumzeit-Verarbeitungsverfahren werden dargestellt, um die menschliche Trajektoriendaten, wie die Verwendung einer GPS-Einrichtung, zum Zweck der Modellierung Fußgängers Raumzeit Tätigkeiten gesammelten analysieren. / Video/4416 &quot;&gt; Murine fetale Echokardiographie Gene H. Kim Department of Medicine, Abteilung für Kardiologie, Institute for Cardiovascular Research, University of Chicago Fetalen und perinatalen Tod ist ein gemeinsames Merkmal bei der Untersuchung genetischer Veränderungen beeinflussen kardiale Entwicklung. Hochfrequenz-Ultraschall-Bildgebung hat 2-D-Auflösung verbessert und kann ausgezeichnete Informationen über frühe Herz-Entwicklung bieten und ist eine ideale Methode, um die Auswirkungen auf die kardiale Struktur und Funktion vor dem Tod zu erkennen.

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february 2013: this month in jove

Hier ist ein Blick darauf, was kommen im Februar 2013 Ausgabe des Journal of Visualized Experiments (Jupiter). Seit der ersten Ausgabe JoVE Jahren hat der biomedizinischen Forschung die überwiegende Anteil unserer Inhalte besteht, und wir haben Teile für Neurowissenschaften, Klinische und Translationale Medizin, Biotechnik und Immunologie. Im Juli 2012 haben wir aus den Naturwissenschaften mit der Einführung JoVE Applied Physics verzweigt. In diesem Monat sind wir erfreut, die erste Video-Artikeln in JoVE Chemie, die Themen reichen von komplexen Biochemie, Assay-Entwicklung, bietet die chemische Verfahrenstechnik, und der organischen Synthese freizugeben. In der chemischen Engineering-Abteilung, kombinieren Pandey et al. Planare Lithographie mit Selbst-Faltung Methoden zur 3-dimensionalen Strukturen durch die Kunst des Origami inspiriert wurde. Durch Einwirkung von Hitze oder Magnetfelder können diese Mikrostrukturen induziert umklappen Objekten werden und be verwendet werden, um Verbrauchsteuern Gewebe - eine Anwendung, die unter nanoskaligen Biopsien in vivo übersetzen könnte. Nun zu Biochemie, präsentieren und Braslavsky Drori ein Experiment, die die Auswirkungen von Eis-bindenden Proteinen auf Eiskristallbildung messen. Durch Videomikroskopie und anschließende Videoanalyse thermische Hysterese-der Unterschied zwischen Einfrieren und Schmelztemperaturen-kann in Gegenwart Frostschutzproteine, die viele Organismen wie Bakterien und Pflanzen vor Frost geschützt bestimmt werden. In JoVE Applied Physics, Baranec et al. streben nach den Sternen in unserer allerersten Astronomie Artikel, der das sichtbare Universum bringt in den Fokus mit dem Robo-AO-Laser adaptiven Optik. Mit Hilfe einer Hochleistungs-Laserstrahl, korrigiert dieses System die ankommenden Lichtwellen, die durch atmosphärische Turbulenz verzerrt bekommen so Gegenständen im Himmel mit Hochauflösung zu sehen ist. Die Autoren planen, diese technisch zu verwendenque, um Planeten außerhalb unseres Sonnensystems zu finden, und machen eine genaue Beobachtungen astronomischer Phänomene. Rückblickend auf der Erde untersuchen Qi und Du, wie menschliche Bewegungen in der räumlichen und zeitlichen Dimensionen verwendet werden, um die Übertragung von Krankheiten zu untersuchen. GPS-fähige Geräte können verwendet werden, um automatisch sammeln menschlichen Bahndaten, die die Autoren unter Verwendung eines neuartigen interaktiven Schnittstelle werden. Die Trajektorien können auch in Google Earth animiert werden. Diese Methoden können bei der Erkennung &quot;hot spots&quot; für die Übertragung von Infektionskrankheiten, wie Grippe. In JoVE Clinical and Translational Medicine präsentiert Kim eine nicht-invasive Methode zur Bildgebung fetalen Mäuseherzen in utero mit hochfrequenten Ultraschall. Dieser Vorgang, der auch als Echokardiographie, können verwendet werden, um normales Herz Struktur und Funktion sowie die Mechanismen der kardiovaskulären Erkrankung zu untersuchen. Es kann sogar für die frühe Diagnose von Herzfehler verwendet werden, und dass das eine leistungsfähigeTool mit vielen wissenschaftlichen und klinischen Anwendungen. Von Visualisierung in die Weiten des Weltraums, zur Analyse menschlicher Bewegungen in Raum und Zeit, Sie haben gerade einen Blick auf neue Inhalte JoVE die für Februar 2013 gefangen. Wie das Universum, wir weiterhin expandieren, und in diesem Monat markiert unserer Ankunft in einer neuen Grenze mit der Einführung der Chemie in JoVE: The Journal of Visualized Experiments.

Watch this Scientific Journal Video about February 2013: This Month in JoVE at JoVE.com

February 2013: This Month in JoVE

Hier ist ein Blick darauf, was kommen im Februar 2013 Ausgabe des Journal of Visualized Experiments (Jupiter).

Februar 2013: Diesen Monat in JoVE

Here's a look at what's coming up in the February 2013 issue of Journal of Visualized Experiments (JoVE).
Since JoVE's first issue, biomedical research ...

Research

JoVE Journal

Biology

8.9K Aufrufe.  Massachusetts Eye and Ear. Hier ist ein Blick darauf, was kommen im Februar 2013 Ausgabe des Journal of Visualized Experiments (Jupiter).

Serie: February 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

August 2012: Diesen Monat in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Forschung

Biologie

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Das Jahr im Rückblick

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...