<a href="https://www.jove.com/video/4189">LabVIEW a comando nanolitri osmometro Novel per le indagini Protein Ice rilegatura</a> Ido Braslavsky 1, 2, Ran Drori 1 1 Istituto di Biochimica, Scienza degli Alimenti e Nutrizione, il Robert H. Smith Facoltà di Agraria, cibo e ambiente, l&#39;Università Ebraica di Gerusalemme, 2 Dipartimento di Fisica e Astronomia, Ohio University Proteine ​​leganti ghiaccio (IBPs), noto anche come proteine ​​antigelo, inibiscono la crescita di ghiaccio e sono un additivo promettente per l&#39;uso nella crioconservazione dei tessuti. Lo strumento principale utilizzato per indagare IBPs è il osmometro nanolitri. Abbiamo sviluppato una casa progettata fase di raffreddamento montato su un microscopio ottico e controllato utilizzando un custom-built routine di LabVIEW. Il osmometro nanolitri qui descritto manipolato la temperatura del campione in un ultra-minuscole. <ahref = &quot;https://www.jove.com/video/50022&quot;&gt; Origami Ispirato Auto-assemblaggio di particelle Fantasia e riconfigurabile Shivendra Pandey 1, Evin Gultepe 1, H. David Gracias 1, 2 1 Dipartimento di Ingegneria chimica e biomolecolare, The Johns Hopkins University, 2 Dipartimento di Chimica, The Johns Hopkins University Descriviamo dettagli sperimentali della sintesi di particelle fantasia e riconfigurabili da due dimensioni (2D) precursori. Questo metodo può essere utilizzato per creare particelle in una varietà di forme tra poliedri e dispositivi di presa a scale di lunghezza che vanno dalla micro alla scala centimetri. <a href="https://www.jove.com/video/50225">Rapid test colorimetrici per distinguere qualitativamente RNA e DNA nei campioni Biomolecolari</a> Jennifer Patterson, Cameron Mura Dipartimento di Chimica, Università della Virginia Una suite di test colorimetrici è descritto per le proteine ​​rapidamente distinguere, RNA, DNA, e la possibilità di ridurre gli zuccheri in campioni biomolecolari potenzialmente eterogenei. <a href="https://www.jove.com/video/4323">Preparazione ed uso di samario diioduro (SMI 2) nella sintesi organica: il ruolo meccanicistico di HMPA e Ni (II) sali nella reazione Samario Barbier</a> Dhandapani V. Sadasivam, Kimberly Choquette A., Robert A. Fiori II Dipartimento di Chimica, Lehigh University Una procedura semplice per la preparazione di samario diioduro (SMI 2) in THF viene descritto. Il ruolo dei due principali additivi e cioè esametilfosforammide (HMPA) e Ni (ACAC) 2 nelle reazioni mediate Sm è dimostrato nella Sm-Barbier reazione. <a href="https://www.jove.com/video/50021">Portare</a>L&#39;universo visibile in Focus con Robo-AO Christoph Baranec 1, 2, Riddle Reed 1, Nicola Legge M. 3, UN Ramaprakash 4, Shriharsh P. Tendulkar 2, Khanh Bui 1, Mahesh P. Burse 4, Pravin Chordia 4, Hillol K. Das 4, Jack TC Davis 1 , Richard G. Dekany 1, Mansi M. Kasliwal 5, Shrinivas R. Kulkarni 1, 2, Timothy D. Morton 2, Eran O. Ofek 6, Sujit Punnadi 4 1 Caltech Osservatori ottici, California Institute of Technology, 2 Dipartimento di Astronomia, California Institute of Technology, 3 Dunlap Institute for Astronomy and Astrophysics, University of Toronto, 4 Centro interuniversitario per Astronomy &amp; Astrophysics, 5 Osservatori della Carnegie Institution for Science , 6 Benoziyo Center for Astrophysics, Weizmann<html> Istituto di Scienze Luce da oggetti astronomici deve viaggiare attraverso l&#39;atmosfera turbolenta della terra prima di essere ripreso da telescopi terrestri. Per consentire l&#39;imaging diretto alla massima risoluzione teorica angolare, tecniche avanzate come quelle impiegate dal Robo-AO-ottica adattiva sistema deve essere utilizzato. <a href="https://www.jove.com/video/50130">Le analisi per i dati di traiettoria pedonale Lo spazio-tempo di studio di attività</a> Feng Qi 1, Fei du 2 1 Scuola di Ambientali e Scienze della Vita, Kean University, 2 Dipartimento di Geografia, Università di Wisconsin-Madison Una serie di metodi di elaborazione spazio-temporali vengono presentati per analizzare dati di traiettoria umani, come quelli raccolti utilizzando un dispositivo GPS, al fine di modellazione pedonali spazio-temporali attività. / Video/4416 &quot;&gt; L&#39;ecocardiografia fetale murino Gene H. Kim Dipartimento di Medicina, Sezione di Cardiologia, Istituto per la Ricerca Cardiovascolare, Università di Chicago Morte fetale e perinatale è una caratteristica comune quando si studiano le alterazioni genetiche che influenzano lo sviluppo cardiaco. Alta frequenza ecografia ha migliorato 2-D risoluzione e può fornire informazioni excellent precoce sviluppo cardiaco ed è un metodo ideale per rilevare l&#39;impatto sulla struttura e funzione cardiaca prima della morte.

Nessun conflitto di interessi dichiarati.

february 2013: this month in jove

Ecco uno sguardo a ciò che è in arrivo nel numero 2013 febbraio del Journal of Experiments Visualizzato (Giove). Dal primo numero di Giove, la ricerca biomedica ha compreso la frazione predominante dei nostri contenuti, e abbiamo sezioni per Neuroscienze, Medicina Clinica e Traslazionale, Bioingegneria, e Immunologia. Nel mese di luglio 2012, ramificata per le scienze fisiche con il lancio di Giove Fisica Applicata. Questo mese, siamo lieti di rilasciare i primi video-articoli in Chimica JoVE, che dispone di argomenti che vanno dalla biochimica complessa, allo sviluppo, al test di ingegneria chimica, e la sintesi organica. Nel dipartimento di ingegneria chimica, Pandey et al. Combinano litografia planare con l&#39;auto-pieghevoli metodi per creare tre strutture tridimensionali ispirati all&#39;arte degli origami. Attraverso l&#39;esposizione a campi magnetici o calore, queste microstrutture possono essere indotte a ripiegare su oggetti, e può be usato per il tessuto delle accise - un&#39;applicazione che potrebbe tradursi a prendere biopsie su scala nanometrica in vivo. Passando alla biochimica, Braslavsky e Drori presentare un esperimento progettato per misurare gli effetti di ghiaccio proteine ​​leganti sulla formazione di cristalli di ghiaccio. Attraverso la microscopia video e analisi video successivo, isteresi termica, la differenza tra la temperatura di congelamento e di fusione, può essere determinata in presenza di proteine ​​antigelo che proteggono molti organismi come batteri e piante dal gelo. In JoVE Fisica Applicata, Baranec et al. mirare le stelle nel nostro articolo astronomia primo, che porta l&#39;universo visibile a fuoco con il Robo-AO sistema laser di ottica adattiva. Con l&#39;aiuto di un potente raggio laser, questo sistema corregge le onde di luce in entrata che vengono distorte dalla turbolenza atmosferica così oggetti nel cielo può essere visto con alta risoluzione. Gli autori in programma di utilizzare questa tecnicique di trovare pianeti oltre il nostro Sistema Solare, e fare osservazioni precise dei fenomeni astronomici. Guardando indietro sulla Terra, Qi e Du esaminare come i movimenti umani in dimensioni spaziali e temporali possono essere utilizzati per studiare la trasmissione della malattia. GPS dispositivi abilitati possono essere utilizzati per raccogliere automaticamente i dati di traiettoria umani, che il processo autori utilizzando un&#39;interfaccia romanzo interattivo. Le traiettorie possono essere animati in Google Earth. Questi metodi possono aiutare a individuare i &quot;punti caldi&quot; per la trasmissione di malattie infettive, come l&#39;influenza. In JoVE Medicina clinica e traslazionale, Kim presenta un metodo non invasivo per il cuore di topo di imaging fetale in utero mediante ecografia ad alta frequenza. Questa procedura, chiamata anche ecocardiografia, possono essere utilizzati per studiare la struttura e la funzione cardiaca normale così come i meccanismi di malattia cardiovascolare. Può anche essere utilizzato per la diagnosi precoce di difetti cardiaci, rendendo questo un potentestrumento con molte applicazioni scientifiche e cliniche. Da visualizzare le grandi distanze di spazio, per analizzare i movimenti umani nello spazio e nel tempo, si è appena intravisto di nuovi contenuti di Giove per febbraio 2013. Come l&#39;universo, si continua ad espandersi, e questo mese segna il nostro arrivo in una nuova frontiera con il lancio di Chimica in JoVE: The Journal of esperimenti visualizzati.

Watch this Scientific Journal Video about February 2013: This Month in JoVE at JoVE.com

February 2013: This Month in JoVE

Ecco uno sguardo a ciò che è in arrivo nel numero 2013 febbraio del Journal of Experiments Visualizzato (Giove).

Febbraio 2013: Questo mese a JoVE

Here's a look at what's coming up in the February 2013 issue of Journal of Visualized Experiments (JoVE).
Since JoVE's first issue, biomedical research ...

Research

JoVE Journal

Biology

8.9K Views.  Massachusetts Eye and Ear. Ecco uno sguardo a ciò che è in arrivo nel numero 2013 febbraio del Journal of Experiments Visualizzato (Giove).

Video: February 2013: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Agosto 2012: Questo mese in JOVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Ricerca

Biologia

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: A Year in Review

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...