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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

13.1K Views

08:38 min

March 3rd, 2015

March 3rd, 2015

13,148 Views

0:05

Title

1:36

Pin-tool Sterilization and Printing Procedures

2:17

Condensation of the DHFR F[3] Collection in 1,536 Arrays Using an Automated Robotic Platform

4:28

High-throughput DHFR-PCA Procedure and Image Analysis

6:58

Results: Representative Data Analyses

8:08

Conclusion

Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

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