Name: assessment of maternal vascular remodeling during pregnancy in the mouse uterus
Uploaded: 2015-12-05T15:00:00
Duration: 6 min 55 s
Description: Watch this Scientific Journal Video about Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus at JoVE.com

The authors would like to thank all members of the Colucci lab for helpful discussions. This work was funded by The Wellcome Trust [094073/Z/10/Z], The Centre for Trophoblast Research and The British Heart Foundation.

All procedures discussed in this manuscript are in accordance with UK Home Office regulations and are approved by the Cambridge Ethical Review Panel.
1. Specimen Collection
<ol>
	<li>Set up timed matings using 8-12 week old female C57BL/6 mice with adult males (up to 2 females per male) in the afternoon. Check for the presence of vaginal plugs as a sign of copulation early in the morning on the following days. The presence of a plug in the morning marks gd0.5.</li>
	<li>On gd9.5, euthanize the animal by cervical dislocation and open the abdominal cavity. Note that euthanasia by CO2 may cause vasodilatation.</li>
	<li>Use dental floss to gently ligate the uterine arteries (Figure 1, dashed arrows). Tie the floss around the arteries at the tip of each uterine horn, proximal to the ovaries, as well as around the cervix. 
	Note: Do not rupture the vessels as this may result in collapsed arteries.</li>
	<li>Dissect the uterus quickly using dissection scissors, excising the whole uterus distally to the three ligation points at the tip of the uterine horns and the cervix.</li>
	<li>Place the uterus on a prepared polystyrene piece so that both horns are aligned along the same long axis in opposite directions from the cervix. Gently stretch it and pin into place using 2-3 25 G needles.</li>
	<li>Immerse the polystyrene into a prepared 50 ml conical tube. Top up to 50 ml with 10% formalin. Fix for 5-6 hr at room temperature.</li>
	<li>Discard the formalin solution and replace with 50 ml 1x phosphate buffered saline (PBS) for 5 min.</li>
	<li>Excise the two uterine horns proximally to the ligation point&#160;at each end and proximally to the cervix in the center, trim away any excess fat around the implantation sites and wash twice in PBS for 5 min to remove traces of formalin.</li>
	<li>Store the samples in 70% ethanol at 4 &#176;C (for up to two weeks) until processing. 
	CAUTION: Ethanol is highly flammable.</li>
	<li>Use an automated processing system using the parameters indicated in Table 1. Embed the horns individually in paraffin so that they can be cut along the plane perpendicular to the long axis of the uterus. This ensures that the maximum area of each implantation site will be exposed for analysis in the middle of the block (Figure 2A). 
	OPTIONAL: This is a convenient time point for randomization of the blocks if desired.</li>
	<li>Using a microtome, cut serial sections of 7 &#181;m thickness from the blocks. Stain one section at every 49 &#181;m interval with hematoxylin and eosin (H&#38;E, see section 2.1). Store the remaining sections at room temperature for SMA staining and other downstream applications.</li>
</ol>
<table fo:keep-together.within-page="1">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:99px;">Solution</td>
			<td style="width:43px;">Time</td>
			<td style="width:97px;">Temperature</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">70% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100% Ethanol</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Xylene</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Xylene</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Xylene</td>
			<td>1 hr</td>
			<td>40 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Paraffin</td>
			<td>1 hr</td>
			<td>63 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Paraffin</td>
			<td>1 hr</td>
			<td>63 &#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Paraffin</td>
			<td>1 hr</td>
			<td>63 &#176;C</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Paraffin</td>
			<td>1 hr</td>
			<td>63 &#176;C</td>
		</tr>
	</tbody>
</table>
Table 1: Settings for automated tissue processing. Overview of the setting used for the processing of uterine tissue
2. Stereological Assessment
<ol>
	<li>H&#38;E Staining
	<ol>
		<li>Deparaffinize sections by immersing slides mounted in a slide rack into a 100% xylene bath for 10 min at room temperature. 
		CAUTION: Xylene is flammable and a suspected carcinogen that is toxic through contact and inhalation. This work must be conducted in a fume hood, using personal protective equipment (PPE).</li>
		<li>Immerse slides in bath containing clean xylene for a further 10 min.</li>
		<li>Sequentially immerse slides in baths containing 100% ethanol, 95% ethanol, 70% ethanol and purified water for 5 min each.</li>
		<li>Immerse slides in 50% Gill No 3 hematoxylin solution, diluted with purified water, for 5 min. 
		CAUTION: Hematoxylin is harmful by ingestion and contact to eyes. Wear appropriate PPE when handling.</li>
		<li>Wash slides in a staining trough under running tap water for 10 min.</li>
		<li>Immerse slides in 1% acid/alcohol solution (1% 10 M hydrochloric acid in 70% ethanol) for 10 sec and wash in a staining trough under running tap water.</li>
		<li>Immerse slides in eosin solution for 30 sec and wash in a staining trough under running tap water for 10 min.</li>
		<li>Within a fume hood, sequentially immerse slides in baths containing 70% ethanol, 95% ethanol and 100% ethanol for 5 min each.</li>
		<li>Immerse slides in bath containing xylene for 10 min.</li>
		<li>Mount coverslips using a xylene-based mounting medium. Dry horizontally and thoroughly in a fume hood prior to visualizing the slides under a microscope.</li>
	</ol>
	</li>
	<li>Stereological Assessment of Vessel Size and Remodeling Status
	<ol>
		<li>For each implantation site, determine which sections are close to the midsagittal point. The chorioallantoic attachment between the fetus and developing placenta serves as a good indicator of the midsagittal point.</li>
		<li>Select 3 sections at 49 &#181;m intervals close to the midsagittal point for the analysis. Scan selected slides. In order to avoid including veins, which have been shown to be more peripherally located in the implantation sites16, restrict the analysis on the central 2/4 of each implantation site (Figure 2A).</li>
		<li>To assess lumen size, draw around the inside of the vessels and record the area of this shape. For the total vessel size, draw around the outer wall of the vessel, including endothelial cells, pericytes and intramural leukocytes. 
		Note: The ratio of total vessel size to lumen is a proxy for the remodeling status of a vessel. As the vessels coil through the plane that the section was cut along, there may be multiple cross-sections of the same artery in one slide. Avoid measuring the same artery multiple times.</li>
		<li>Record the measurements from the 5 vessels in each implantation site with the largest and roundest lumens. Measure each implantation site in triplicate (three sections spaced 49 &#181;m apart from each other). The mean of these 15 measurements represents the mean diameter of the largest vessels.</li>
		<li>To avoid overrepresentation of larger vessels in some samples, measure the same number of vessels in each implantation site.</li>
	</ol>
	</li>
</ol>
3. Qualitative Assessment Using Immunohistochemistry
<ol>
	<li>Deparaffinization and Rehydration
	<ol>
		<li>Deparaffinize sections by immersing slides mounted in a heatproof slide rack into a 100% xylene bath for 10 min at room temperature.</li>
		<li>Immerse slides in bath containing clean xylene for a further 10 min.</li>
		<li>Sequentially immerse slides in baths containing 100% ethanol, 95% ethanol and 70% and purified water for 5 min each.</li>
	</ol>
	</li>
	<li>Antigen Retrieval
	<ol>
		<li>Prepare a 10 mM sodium citrate buffer in purified water and adjust to pH 6 using 10 M hydrochloric acid.</li>
		<li>Fill a heatproof container that can hold 600 ml with 400 ml sodium citrate buffer and place in a domestic pressure cooker containing sufficient water to submerge half of the container. Loosely attach the lid of the pressure cooker and heat until boiling.</li>
		<li>Place slide rack into the sodium citrate buffer and tightly fix the lid of the pressure cooker. Once pressurized, allow to boil for 3 min.</li>
		<li>Depressurize the pressure cooker and remove the inner container. Allow slides to cool in the sodium citrate buffer for 10 min at room temperature.</li>
		<li>Wash slides in a bath containing purified water for 5 min.</li>
		<li>Encircle the sections using a hydrophobic barrier pen.</li>
		<li>Wash slides by submerging in PBS for 5 min.</li>
	</ol>
	</li>
	<li>Blocking Endogenous Peroxidase and Non-specific Binding
	<ol>
		<li>Incubate sections with 3% hydrogen peroxide diluted in purified water in a humidified chamber for 30 min at room temperature. 
		CAUTION: Hydrogen peroxide is highly irritant to eyes, skin and upon ingestion. Wear appropriate PPE.</li>
		<li>Tap off excess hydrogen peroxide solution and wash slides twice by submerging in Tris buffered saline (TBS) for 2 min each.</li>
		<li>Incubate sections with mouse immunoglobulin blocking reagent from the mouse on mouse kit prepared according to manufacturer&#39;s instructions.</li>
		<li>Wash slides twice in TBS for 2 min each.</li>
	</ol>
	</li>
	<li>Immunodetection of Smooth Muscle Actin
	<ol>
		<li>Prepare antibody diluent solution (provided in kit) and incubate with sections for 5 min at room temperature, or according to manufacturer&#39;s instructions.</li>
		<li>Dilute 1:100 mouse anti-human smooth muscle actin (DAKO M0851) in antibody diluent solution. Dilute mouse IgG2a isotype control in antibody diluent solution, ensuring that the final immunoglobulin concentration is equivalent in both solutions.</li>
		<li>Tap off excess antibody diluent solution and cover sections with either diluted antibody or isotype control solutions. Incubate in a humidified chamber for 30 min at room temperature.</li>
		<li>Wash slides twice in TBS for 2 min each.</li>
		<li>Dilute biotinylated anti-mouse secondary antibody and incubate sections for 10 min at room temperature, or according to manufacturers instructions.</li>
		<li>Wash slides once each with TBS and PBS for 2 min, respectively.</li>
		<li>Prepare 3,3&#39;-Diaminobenzidine (DAB) solution according to manufacturer&#39;s instructions. Cover sections with DAB solution and incubate for up to 4 min, ensuring to monitor sections to avoid excess color development. 
		CAUTION: DAB is flammable and a suspected carcinogen that is toxic through contact and inhalation. Wear appropriate PPE when handling.</li>
		<li>Immerse slides in purified water once desired intensity of staining is reached.</li>
		<li>Counterstain with 50% Gill No. 3 hematoxylin for 20 sec&#160;and wash in a staining trough under running tap water until water runs clear.</li>
	</ol>
	</li>
	<li>Dehydration and Mounting
	<ol>
		<li>Within a fume hood, sequentially immerse slides in baths containing 70% ethanol, 95% ethanol and 100% ethanol for 5 min each.</li>
		<li>Immerse slides in bath containing xylene for 10 min.</li>
		<li>Mount coverslips using a xylene-based mounting medium. Dry horizontally and thoroughly in a fume hood prior to visualizing the slides under a microscope</li>
	</ol>
	</li>
</ol>

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The authors have nothing to disclose.

The protocol presented here is designed to provide a reproducible method by which to assess the vascular changes that are necessary for successful pregnancy. Critical for the success of this evaluation is the quality of the tissue sections. Both accurately determining the gestational age of the implantation sites as well as reliably ligating the uterine arteries are crucial steps. Changes in parameters such as time of fixation, tissue-processing protocol, etc. may also affect the result. For an unbiased stereological assessment, it is important to measure the same number of vessels in each implantation site. It is recommended to measure 5 vessels per implantation site and each implantation site in triplicate. The mean of these 15 measurements represents the mean size of the 5 largest vessels.
Further to the data from alymphoid Rag2-/-IL2rg-/- mice shown here, we found this protocol useful for a number of models of inadequate maternal immune function, including one of inhibited NK cells14. Defects in arterial remodeling have also been shown in models of impaired trophoblast invasion18 and the techniques described here may be helpful to assess the vasculature in these cases. We optimized and validated this protocol for the investigation of decidual vascular remodeling at mid-gestation in mouse, but it is also conceivable to adapt this protocol to work in other model systems (e.g. rats) or even in other organs. While we find that the NK-dependent remodeling can be robustly assessed at gd9.5, it may be amended for other time points such as gd12.5, when the maternal vasculature is completely remodeled. At this time point, endovascular trophoblast invasion is deeper16 and this time point may be more suitable for investigations of the role of trophoblast for vascular changes. If additional quantitative readouts are desired, investigators can also increase the number of sections stained for SMA and assess the percentage of partially remodeled vessels within each implantation site.
A limitation of this protocol is the descriptive nature of histological examinations. Functional assays, however, are only slowly becoming available (such as Doppler ultrasound19). These techniques require technical expertise and much higher expenses for acquiring and maintaining equipment, but have the advantage of providing a longitudinal in vivo readout for the effect of the changes that can be observed histologically. A possible drawback of all stereological examinations is the subjectivity of the investigators. To this end, using two independent examiners that analyze all samples independently can help to avoid this issue. While the protocol outlined here gives insight into how much blood can reach the fetomaternal interface, it may be advantageous to combine it with the complementary approach of assessing the total amount of blood at any given time in the vasculature through plastic casts16.
The strength of this protocol is that it combines two independent approaches. Vascular change is first quantitatively assessed by stereology and the result is then validated qualitatively using immunohistochemistry. The reliability of the results can be further strengthened by randomizing the samples. The samples prepared for this protocol can easily be used to also investigate other parameters of pregnancy such as decidualisation, development of the mesometrial lymphoid aggregate of pregnancy (MLAp) or immunodetection of cells of interest, to rigorously assess a pregnancy phenotype at midgestation.
Arterial remodeling is a critical step for uncomplicated pregnancies in women and failure of these changes underpins the great obstetrical syndromes (GOS), namely pre-eclampsia, fetal growth restriction and stillbirth. We present here techniques to carefully assess a pregnancy phenotype in mouse models that mimic some characteristics of the pathophysiology of GOS.

The exchange of nutrients, gases and waste products during eutherian gestation is mediated by the placenta. In the female reproductive tract, the uterine arteries are the main conduits of blood to the uterus. After implantation, these branch into specialized vessels called spiral arteries that coil through the decidua basalis towards the fetoplacental unit. Diameter and elasticity of these spiral arteries, which are surrounded by leukocytes, in particular uterine natural killer (uNK) cells, dictate volume and velocity of blood available to the placenta1,2. Correct hemodynamic changes as a result of remodeling of the spiral arteries are critical for pregnancy success.
While the underlying mechanisms differ in detail between human and murine pregnancy, the final result of the remodeling process in both species is dilated, high conductance vasculature that loses its smooth muscle layer. In mice, uNK cell-derived interferon (IFN-)&#947; is necessary to induce these changes at around mid-gestation3-5. Mice that lack either NK cells or components of the IFN-&#947; signaling pathway fail to undergo these changes and this is associated with reduced fetal growth6,7, stressing the importance of an adequate blood supply to the fetoplacental unit. During early human pregnancy, the interstitial and endovascular invasion of extravillous trophoblast and their interaction with uNK cells are important for inducing vascular changes and promoting fetal growth8-10. uNK cells can orchestrate human trophoblast invasion through chemokines11 and cytokines such as granulocyte macrophage - colony stimulating factor (GM-CSF)12. Shallow trophoblast invasion is associated with reduced arterial remodeling and pregnancy complications such as pre-eclampsia9.
This protocol is based on the pioneering work of Anne Croy who described the important role of the immune system and particularly NK-derived IFN-&#947; for successful vascular remodeling through immunohistochemistry and elegant transfer experiments5,13. Here we describe a fast and economical way to assess the remodeling status of spiral arteries. Although our lab routinely assesses this at mid-gestation (gestation day (gd)9.5)14, the remodeling process takes place between gd8.5 and 12.515. The advent of automated slide scanners has greatly facilitated the assessment of vessel and lumen areas from sections and we found this to be a more reproducible approach than measuring vessel diameters. The addition of immunohistochemical detection of SMA allows for a straightforward validation of the results obtained from the stereological assessment. As with all stereological techniques, it is recommended to perform the assessment as a blinded experiment by at least two examiners. To this end, a randomization step can be introduced when serially cutting the samples so that the investigators assessing the slides do not know from which experimental group the samples come.
The overall goal of this protocol is to provide a reliable tool to carefully assess the remodeling of spiral arteries in mice with defined maternal and paternal genotypes, in the context of mouse models for pregnancy-related complications. The results stress the dependence on uNK cells of this process, which is essential for normal fetal growth.

<table border="0" cellpadding="0" cellspacing="0" width="704">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">C57BL/6 mice (8-12 weeks old)</td>
			<td style="width:99px;">Charles River</td>
			<td style="width:111px;"></td>
			<td style="width:283px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Dental floss</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Polystyrene</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td>Used for holding tissue in place</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Straight dissection scissors</td>
			<td style="width:99px;">EMS</td>
			<td style="width:111px;">72940</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">25G needles</td>
			<td style="width:99px;">BD&#160;</td>
			<td style="width:111px;">300600</td>
			<td>Used for holding tissue in place</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">50 ml conical tubes</td>
			<td style="width:99px;">Falcon</td>
			<td style="width:111px;">352070</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Formalin solution, neutral buffered, 10%</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">HT501128-4L</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Phosphate buffered saline</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">P3813-10PAK</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Ethanol</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">32221-2.5L</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Paraffin</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">327204-1KG</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Xylene</td>
			<td style="width:99px;">Fisher Scientific</td>
			<td style="width:111px;">X/0250/17</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Automated tissue processor</td>
			<td style="width:99px;">Sakura</td>
			<td style="width:111px;">6032</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Microtome</td>
			<td style="width:99px;">Leica</td>
			<td style="width:111px;">RM2245</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Slide rack</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">Z710989</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Coplin jar</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">S5891</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Purified water</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Hematoxylin solution, Gill No. 3</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">GHS332-1L</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Eosin Y solution</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">HT110116-500ML</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Fume hood</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Xylene-based mounting media</td>
			<td style="width:99px;">VWR</td>
			<td style="width:111px;">361254D</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Slide scanner</td>
			<td style="width:99px;">Hamamatsu</td>
			<td style="width:111px;">NanoZoomer</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Slide scanner software</td>
			<td style="width:99px;">Hamamatsu</td>
			<td style="width:111px;">NDP viewer</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Sodium citrate tribasic dihydrate</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">32320</td>
			<td>Make 10 mM solution and adjust pH to 6</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Pressure cooker</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td>Used for antigen retrieval</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Heating plate</td>
			<td style="width:99px;"></td>
			<td style="width:111px;"></td>
			<td>Used for antigen retrieval</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Hydrophobic barrier pen</td>
			<td style="width:99px;">Vector</td>
			<td style="width:111px;">H-4000</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Hydrogen peroxide</td>
			<td style="width:99px;">Fisher Scientific</td>
			<td style="width:111px;">H/1800/15</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Tris buffered saline</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">T6664-10PAK</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Mouse on mouse kit</td>
			<td style="width:99px;">Vector</td>
			<td style="width:111px;">BMK-2202</td>
			<td>Significantly reduces background</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Mouse anti-human smooth muscle actin</td>
			<td style="width:99px;">DAKO</td>
			<td style="width:111px;">M0851</td>
			<td>Cross-reacts with mouse</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">IgG2a isotype control</td>
			<td style="width:99px;">DAKO</td>
			<td style="width:111px;">X0943</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:212px;">Humidified chamber</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">H6644</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">3,3&#8217;-Diaminobenzidine solution</td>
			<td style="width:99px;">Sigma</td>
			<td style="width:111px;">D4293-5SET</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:212px;">Hydrochloric acid</td>
			<td style="width:99px;">Fisher Scientific</td>
			<td style="width:111px;">H/1050/PB17</td>
			<td></td>
		</tr>
	</tbody>
</table>

assessment of maternal vascular remodeling during pregnancy in the mouse uterus

The placenta mediates the exchange of factors such as gases and nutrients between mother and fetus and has specific demands for supply of blood from the maternal circulation. The maternal uterine vasculature needs to adapt to this temporary demand and the success of this arterial remodeling process has implications for fetal growth. Cells of the maternal immune system, especially natural killer (NK) cells, play a critical role in this process. Here we describe a method to assess the degree of remodeling of maternal spiral arteries during mouse pregnancy. Hematoxylin and eosin-stained tissue sections are scanned and the size of the vessels analysed. As a complementary validation method, we also present a qualitative assessment for the success of the remodeling process by immunohistochemical detection of smooth muscle actin (SMA), which normally disappears from within the arterial vascular media at mid-gestation. Together, these methods enable determination of an important parameter of the pregnancy phenotype. These results can be combined with other endpoints of mouse pregnancy to provide insight into the mechanisms underlying pregnancy-related complications.

Rag2-/-IL2rg-/- mice lack all mature lymphocytes, including NK cells17, and their uterine arteries fail to undergo the vascular changes seen in congenic wildtype C57BL/6 (WT) mice around mid-gestation5,14. As a result of this reduced remodeling Rag2-/-IL2rg-/- mice show significantly smaller luminal surface areas, indicative of overall smaller vessels which can be visualized on H&#38;E-stained sections and quantified stereologically (Figure 2). Furthermore, the relative wall thickness (vessel to lumen ratio) is higher in the spiral arteries in these mice, suggesting reduced supply of blood and higher blood velocity.
This quantitative assessment was confirmed using immunohistochemical detection of SMA. It is characteristic for WT mice to lose most of the SMA within the vascular media of the spiral arteries by mid-gestation. If this NK cell-driven process does not take place, SMA remains in the media of the vessels and can be readily detected immunohistochemically as rings around the blood vessels (Figure 3).
<img alt="Figure 1" src="/files/ftp_upload/53534/53534fig1.jpg" /> 
Figure 1: Mouse uterus at gd9.5, schematic overview. Drawing showing the relative positions of the uterine horns, cervix (blue) and main uterine arteries (red). Indicated are the long axis of the uterine horns (arrows) and the ligation points (dashed arrows). The sections for immunohistochemistry are cut along the plane of view.
<img alt="Figure 2" src="/files/ftp_upload/53534/53534fig2.jpg" />
Figure 2: Stereological assessment of arterial remodeling at mid-gestation. (A) Example assessment of luminal and total vessel areas on H&#38;E stained sections from WT females in the central 2/4 of the decidua basalis (demarcated by the vertical black dashed lines) at gd9.5. In the vessels at higher magnification, the red dashed line demarcates total vessel area, yellow dashed line demarcates lumen area. Bar = 500 &#181;m (left), 50 &#181;m (right). The long axis of the uterus is a horizontal line in this orientation. (B) Quantification of luminal areas (left panel) and ratio of luminal area to total vessel area (right panel) from WT (C57BL/6) and Rag2-/-Il2rg-/- mice (on C57BL/6 background). Each data point represents the mean of 15 measurements (5 measurements per section, 3 sections per implantation site). Bars represent mean of n = 11-13 implantation sites from 4 pregnancies per group; p-value from a two-tailed, unpaired Mann-Whitney test. WT: wildtype.
<img alt="Figure 3" src="/files/ftp_upload/53534/53534fig3.jpg" /> 
Figure 3: Immunohistochemical detection of smooth muscle actin in the decidua basalis. Representative SMA staining on WT (top) and Rag2-/-IL2rg-/- mice (on C57BL/6 background, bottom) at gd9.5. Bar = 100 &#181;m. WT: wildtype.

Watch this Scientific Journal Video about Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus at JoVE.com

Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus

This protocol describes a technique to assess changes in the maternal vasculature during pregnancy in mice. Using stereological methods, remodeling of the decidual spiral arteries is assessed quantitatively and the results confirmed qualitatively using immunohistochemistry.

The placenta mediates the exchange of factors such as gases and nutrients between mother and fetus and has specific demands for supply of blood from the ...

assessment-maternal-vascular-remodeling-during-pregnancy-mouse

Research

JoVE Journal

Developmental Biology

Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish

This protocol introduces how to detect differentiated interrenal steroidogenic cells through a simple whole-mount enzymatic activity assay. Identifying differentiated steroidogenic tissues through chromogenic histochemical staining of 3-&#946;-Hydroxysteroid dehydrogenase /&#916;5-4 isomerase (3&#946;-Hsd) activity-positive cells is critical for monitoring the morphology and differentiation of adrenocortical and interrenal tissues in mammals and teleosts, respectively. In the zebrafish model, the optical transparency and tissue permeability of the developing embryos and larvae allow for whole-mount staining of 3&#946;-Hsd activity. This staining protocol, as performed on transgenic fluorescent reporter lines marking the developing pronephric and endothelial cells, enables the detection of the steroidogenic interrenal tissue in addition to the kidney and neighboring vasculature. In combination with vibratome sectioning, immunohistochemistry, and confocal microscopy, we can visualize and assay the vascular microenvironment of interrenal steroidogenic tissues. The 3&#946;-Hsd activity assay is essential for studying the cell biology of the zebrafish interrenal gland because to date, no suitable antibody is available for labeling zebrafish steroidogenic cells. Furthermore, this assay is rapid and simple, thus providing a powerful tool for mutant screens targeting adrenal (interrenal) genetic disorders as well as for determining disruption effects of chemicals on steroidogenesis in pharmaceutical or toxicological studies.

The interrenal gland in zebrafish is the teleostean counterpart of the mammalian adrenal gland. This protocol introduces how to perform the 3-&#946;-hydroxysteroid dehydrogenase (&#916;5-4 isomerase; 3&#946;-Hsd) enzymatic activity assay, which detects differentiated steroidogenic cells in the developing zebrafish.

This protocol introduces how to detect differentiated interrenal steroidogenic cells through a simple whole-mount enzymatic activity assay. Identifying ...

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Mouse embryonic stem cells (ESCs) isolated from the inner mass of the blastocyst (typically at day E3.5), can be used as in vitro model system for studying early embryonic development. In the absence of leukemia inhibitory factor (LIF), ESCs differentiate by default into neural precursor cells. They can be amassed into a three dimensional (3D) spherical aggregate termed embryoid body (EB) due to its similarity to the early stage embryo. EBs can be seeded on fibronectin-coated coverslips, where they expand by growing two dimensional (2D) extensions, or implanted in 3D collagen matrices where they continue growing as spheroids, and differentiate into the three germ layers: endodermal, mesodermal, and ectodermal. The 3D collagen culture mimics the in vivo environment more closely than the 2D EBs. The 2D EB culture facilitates analysis by immunofluorescence and immunoblotting to track differentiation. We have developed a two-step neural differentiation protocol. In the first step, EBs are generated by the hanging-drop technique, and, simultaneously, are induced to differentiate by exposure to retinoic acid (RA). In the second step, neural differentiation proceeds in a 2D or 3D format in the absence of RA.

We describe a technique for using murine embryonic stem cells for generating two or three dimensional embryoid bodies. We then explain how to induce neural differentiation of the embryoid body cells by retinoic acid, and how to analyze their state of differentiation by progenitor cell marker immunofluorescence and immunoblotting.

Mouse embryonic stem cells (ESCs) isolated from the inner mass of the blastocyst (typically at day E3.5), can be used as in vitro model system for ...

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Endothelial cells (ECs) are the fundamental building blocks of the vascular architecture and mediate vascular growth and remodeling to ensure proper vessel development and homeostasis. However, studies on endothelial lineage hierarchy remain elusive due to the lack of tools to gain access as well as to directly evaluate their behavior in vivo. To address this shortcoming, a new tissue model to study angiogenesis using the mammary fat pad has been developed. The mammary gland develops mostly in the postnatal stages, including puberty and pregnancy, during which robust epithelium proliferation is accompanied by extensive vascular remodeling. Mammary fat pads provide space, matrix, and rich angiogenic stimuli from the growing mammary epithelium. Furthermore, mammary fat pads are located outside the peritoneal cavity, making them an easily accessible grafting site for assessing the angiogenic potential of exogenous cells. This work also describes an efficient tracing approach using fluorescent reporter mice to specifically label the targeted population of vascular endothelial stem cells (VESCs) in vivo. This lineage tracing method, coupled with subsequent tissue whole-mount microscopy, enable the direct visualization of targeted cells and their descendants, through which the proliferation capability can be quantified and the differentiation commitment can be fate-mapped. Using these methods, a population of bipotent protein C receptor (Procr) expressing VESCs has recently been identified in multiple vascular systems. Procr+ VESCs, giving rise to both new ECs and pericytes, actively contribute to angiogenesis during development, homeostasis, and injury repair. Overall, this manuscript describes a new mammary fat pad transplantation and in vivo lineage tracing techniques that can be used to evaluate the stem cell properties of VESCs.

This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.

Endothelial cells (ECs) are the fundamental building blocks of the vascular architecture and mediate vascular growth and remodeling to ensure proper ...

Live Imaging of Mouse Secondary Palate Fusion

The fusion of the secondary palatal shelves to form the intact secondary palate is a key process in mammalian development and its disruption can lead to cleft secondary palate, a common congenital anomaly in humans. Secondary palate fusion has been extensively studied leading to several proposed cellular mechanisms that may mediate this process. However, these studies have been mostly performed on fixed embryonic tissues at progressive timepoints during development or in fixed explant cultures analyzed at static timepoints. Static analysis is limited for the analysis of dynamic morphogenetic processes such a palate fusion and what types of dynamic cellular behaviors mediate palatal fusion is incompletely understood. Here we describe a protocol for live imaging of ex vivo secondary palate fusion in mouse embryos. To examine cellular behaviors of palate fusion, epithelial-specific Keratin14-cre was used to label palate epithelial cells in ROSA26-mTmGflox reporter embryos. To visualize filamentous actin, Lifeact-mRFPruby reporter mice were used. Live imaging of secondary palate fusion was performed by dissecting recently-adhered secondary palatal shelves of embryonic day (E) 14.5 stage embryos and culturing in agarose-containing media on a glass bottom dish to enable imaging with an inverted confocal microscope. Using this method, we have detected a variety of novel cellular behaviors during secondary palate fusion. An appreciation of how distinct cell behaviors are coordinated in space and time greatly contributes to our understanding of this dynamic morphogenetic process. This protocol can be applied to mutant mouse lines, or cultures treated with pharmacological inhibitors to further advance understanding of how secondary palate fusion is controlled.

Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

The fusion of the secondary palatal shelves to form the intact secondary palate is a key process in mammalian development and its disruption can lead to ...

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

High-frequency ultrasonography (HFUS) is a common method to non-invasively monitor the real-time development of the human fetus in utero. The mouse is routinely used as an in vivo model to study embryo implantation and pregnancy progression. Unfortunately, such murine studies require pregnancy interruption to enable follow-up phenotypic analysis. To address this issue, we used three-dimensional (3-D) reconstruction of HFUS imaging data for early detection and characterization of murine embryo implantation sites and their individual developmental progression in utero. Combining HFUS imaging with 3-D reconstruction and modeling, we were able to accurately quantify embryo implantation site number as well as monitor developmental progression in pregnant C57BL6J/129S mice from 5.5 days post coitus (d.p.c.) through to 9.5 d.p.c. with the use of a transducer. Measurements included: number, location, and volume of implantation sites as well as inter-implantation site spacing; embryo viability was assessed by cardiac activity monitoring. In the immediate post-implantation period (5.5 to 8.5 d.p.c.), 3-D reconstruction of the gravid uterus in both mesh and solid overlay format enabled visual representation of the developing pregnancies within each uterine horn. As genetically engineered mice continue to be used to characterize female reproductive phenotypes derived from uterine dysfunction, this method offers a new approach to detect, quantify, and characterize early implantation events in vivo. This novel use of 3-D HFUS imaging demonstrates the ability to successfully detect, visualize, and characterize embryo-implantation sites during early murine pregnancy in a non-invasive manner. The technology offers a significant improvement over current methods, which rely on the interruption of pregnancies for gross tissue and histopathologic characterization. Here we use a video and text format to describe how to successfully perform ultrasounds of early murine pregnancy to generate reliable and reproducible data with reconstruction of the uterine form in mesh and solid 3-D images.

Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.

High-frequency ultrasonography (HFUS) is a common method to non-invasively monitor the real-time development of the human fetus in utero. The mouse is ...

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Nutrient and gas exchange between mother and fetus occurs at the interface of the maternal intervillous blood and the vast villous capillary network that makes up much of the parenchyma of the human placenta. The distal villous capillary network is the terminus of the fetal blood supply after several generations of branching of vessels extending out from the umbilical cord. This network has a contiguous cellular sheath, the syncytial trophoblast barrier layer, which prevents mixing of fetal blood and the maternal blood in which it is continuously bathed. Insults to the integrity of the placental capillary network, occurring in disorders such as maternal diabetes, hypertension and obesity, have consequences that present serious health risks for the fetus, infant, and adult. To better define the structural effects of these insults, a protocol was developed for this study that captures capillary network structure on the order of 1 - 2 mm3 wherein one can investigate its topological features in its full complexity. To accomplish this, clusters of terminal villi from placenta are dissected, and the trophoblast layer and the capillary endothelia are immunolabeled. These samples are then clarified with a new tissue clearing process which makes it possible to acquire confocal image stacks to z- depths of ~1 mm. The three-dimensional renderings of these stacks are then processed and analyzed to generate basic capillary network measures such as volume, number of capillary branches, and capillary branch end points, as validation of the suitability of this approach for capillary network characterization.

This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

Nutrient and gas exchange between mother and fetus occurs at the interface of the maternal intervillous blood and the vast villous capillary network that ...

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Mammalian females periodically ovulate an almost constant number of oocytes during each estrus cycle. To sustain such regularity and periodicity, regulation occurs at the hypothalamic-pituitary-gonadal axis level and on developing follicles in the ovary. Despite active studies, follicle development mechanisms are not clear because of the several steps involved from the dormant primordial follicle activation to ovulation, and because of the regulation complexity that differs at each follicular stage. To investigate the mechanisms of follicle development, and the dynamics of follicles throughout the estrus cycle, we developed a mouse ovarian tissue culture model that can be used to observe follicle development using a microscope. Systematic follicle development, periodical ovulation, and follicle atresia can all be reproduced in the cultured ovary model, and the culture conditions can be experimentally modulated. Here, we demonstrate the usefulness of this method in the study of the regulatory mechanisms of follicle development and other ovarian phenomena.

Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.

Mammalian females periodically ovulate an almost constant number of oocytes during each estrus cycle. To sustain such regularity and periodicity, ...

Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord

The present study combines in utero transduction with transmission electron microscopy (TEM) aiming at a precise morphometrical analysis of ultrastructural parameters in unambiguously identified topographical structures, affected by a protein of interest that is introduced into the organism via viral transfer. This combined approach allows for a smooth transition from macrostructural to ultrastructural identification by following topographical navigation maps in a tissue atlas. High-resolution electron microscopy of the in-utero-transduced tissue reveals the fine ultrastructure of the neuropil and its plasticity parameters, such as cross-sectioned synaptic bouton areas, the number of synaptic vesicles and mitochondria within a bouton profile, the length of synaptic contacts, cross-sectioned axonal areas, the thickness of myelin sheaths, the number of myelin lamellae, and cross-sectioned areas of mitochondria profiles. The analysis of these parameters reveals essential insights into changes of ultrastructural plasticity in the areas of the nervous system that are affected by the viral transfer of the genetic construct. This combined method can not only be used for studying the direct effect of genetically engineered biomolecules and/or drugs on neuronal plasticity but also opens the possibility to study the in utero rescue of neuronal plasticity (e.g., in the context of neurodegenerative diseases).

The combination of transmission electron microscopy and in utero transduction is a powerful approach for studying morphological changes in the fine ultrastructure of the nervous system during development. This combined method allows deep insights into changes in structural details underlying neuroplasticity with respect to their topographical representation.

The present study combines in utero transduction with transmission electron microscopy (TEM) aiming at a precise morphometrical analysis of ...

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

Blood vessels form intricate networks in 3-dimensional space. Consequently, it is difficult to visually appreciate how vascular networks interact and behave by observing the surface of a tissue. This method provides a means to visualize the complex 3-dimensional vascular architecture of the lung.
To accomplish this, a catheter is inserted into the pulmonary artery and the vasculature is simultaneously flushed of blood and chemically dilated to limit resistance. Lungs are then inflated through the trachea at a standard pressure and the polymer compound is infused into the vascular bed at a standard flow rate. Once the entire arterial network is filled and allowed to cure, the lung vasculature may be visualized directly or imaged on a micro-CT (&#181;CT) scanner.
When performed successfully, one can appreciate the pulmonary arterial network in mice ranging from early postnatal ages to adults. Additionally, while demonstrated in the pulmonary arterial bed, this method can be applied to any vascular bed with optimized catheter placement and endpoints.

The aim of this technique is ex vivo visualization of pulmonary arterial networks of early postnatal and adult mice through lung inflation and injection of a radio-opaque polymer-based compound via the pulmonary artery. Potential applications for casted tissues are also discussed.

Blood vessels form intricate networks in 3-dimensional space. Consequently, it is difficult to visually appreciate how vascular networks interact and ...

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Events associated with oocyte nuclear maturation have been well described. However, much less is known about the molecular pathways and processes that take place in the cytoplasm in preparation for fertilization and acquisition of totipotency. During oocyte maturation, changes in gene expression depend exclusively on the translation and degradation of maternal messenger RNAs (mRNAs) rather than on transcription. Execution of the translational program, therefore, plays a key role in establishing oocyte developmental competence to sustain embryo development. This paper is part of a focus on defining the program of maternal mRNA translation that takes place during meiotic maturation and at the oocyte-to-zygote transition. In this method paper, a strategy is presented to study the regulation of translation of target mRNAs during in vitro oocyte maturation. Here, a Ypet reporter is fused to the 3&#39; untranslated region (UTR) of the gene of interest and then micro-injected into oocytes together with polyadenylated mRNA encoding for mCherry to control for injected volume. By using time-lapse microscopy to measure reporter accumulation, translation rates are calculated at different transitions during oocyte meiotic maturation. Here, the protocols for oocyte isolation and injection, time-lapse recording, and data analysis have been described, using the Ypet/interleukin-7 (IL-7)-3&#39; UTR reporter as an example.

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

Events associated with oocyte nuclear maturation have been well described. However, much less is known about the molecular pathways and processes that ...