Endobronchial Biopsy Enzymatic Digestion and Flow Cytometric Analysis
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Results: Identifying Immune and Dendritic Cell Subsets in Endobronchial Biopsies
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Conclusion
Transkript
The main advantage of this technique is that a lot of information can be generated from small tissues obtained during bronchoscopy.This method can help answer key questions in mucosal immunology, where bronchoscopy is part of establishing the diag
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Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.