Sealing the Wells with an Agarose Coated Coverslip and Imaging
4:53
Bacterial Growth Analysis
7:10
Results: Growth of P. aeruginosa Mutants
8:28
Conclusion
Transkript
The overall goal of this method is high throughput parallel analysis of multi-member microbial communities in confined environments using silicon micro well arrays. The main advantage of this technique is that it allows for a high throughput highl
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The development of microbial communities depends on a combination of factors, including environmental architecture, member abundance, traits, and interactions. This protocol describes a synthetic, microfabricated environment for the simultaneous tracking of thousands of communities contained in femtoliter wells, where key factors such as niche size and confinement can be approximated.