Results: Proper Puromycin Treatment and Analysis in MRC-5 and Other Cell Types
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Conclusion
Transkript
The overall goal of this experiment is to visualize and quantify compartmentalized translation events taking place during the initial steps of cell adhesion. This method has broad application. Use it whenever rapid specific translation response mi
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This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.