Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

The overall goal of this procedure is to perform continuous live imaging and single cell tracking of multiple neural cell populations.Followed by post-imaging immunocytochemistry, this method allows the analysis of any cellular events that take place within the track time period.This method can help to answer key questions in the neuroscience field, such as cell behavior and lineage progression of multiple neural population as well as the identification of their modulators.The main advantage of live imaging and single cell tracking in real time is that it allows to monitor variations in cell biology that may pass unnoticed in approaches based on end point analysis.This demonstration will guide the researchers through main steps that can be easily adapted to any culture condition and acquisition software.My name is Rosa Gomez, and I'll be demonstrating the plating and post-imaging immunocytochemistry steps.My name is David Agust