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Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres

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09:31 min

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January 29th, 2018

DOI :

10.3791/57016-v

January 29th, 2018

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7,463 Views

Flor M. Mendez¹, Felipe J. Núñez^1,2, Rocío I. Zorrilla-Veloz^3,4, Pedro R. Lowenstein^1,2, Maria G. Castro^1,2

¹Department of Cell and Developmental Biology, University of Michigan Medical School, ²Department of Neurosurgery, University of Michigan Medical School, ³Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program, University of Michigan Medical School, ⁴Department of Biology, University of Puerto Rico-Río Piedras Campus

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Keywords Native Chromatin Immunoprecipitation

Murine Brain Tumor

Tumor Cells Transcriptome

Antibody Specificity

Fluorescent Protein

Cell Detachment

Neurosphere Formation

Micrococcal Nuclease

Chromatin Digestion

Immunoprecipitation

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